Lipogenesis in the developing brain from intracranially administered (1-/sup 14/C) acetate and (U--/sup 14/C) glucose

Journal Article · Sat Jan 01 04:00:00 EST 1977 · Lipids; (United States)

OSTI ID:6471069

Dhopeshwarkar, G A; Subramanian, C

Fifteen-day-old rats divided into two groups were fiven (1-/sup 14/C) acetate or (U--/sup 14/C) glucose by intracranial injection and were sacrificed after 1 hr. Analysis of lipids from the two groups showed differences in the incorporation of radioactivity in the polar lipids and cholesterol. Analysis of brain fatty acid showed that whereas radioactivity from acetate was incorporated into saturated, mono- and polyunsaturated fatty acids, the radioactivity from (U--/sup 14/C) glucose was found only in 16:0, 18:0, and 18:1. No radioactivity was found in polyunsaturated fatty acids even after concentration on this fraction by AgNO/sub 3/:SiO/sub 2/ thin layer chromatographic method. This difference is discussed in hypothetical terms of nonhomogeneous acetyl CoA pool, formation of acetyl CoA from glucose exclusively inside the mitochondria, and activation of injected acetate to acetyl CoA.

• Research Organization: Univ. of California, Los Angeles
• OSTI ID: 6471069
• Journal Information: Lipids; (United States), Journal Name: Lipids; (United States) Vol. 12:9; ISSN LPDSA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ACETATES
ALDEHYDES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
BRAIN
CARBOHYDRATES
CARBON 14
CARBON ISOTOPES
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
CHEMICAL ANALYSIS
CHOLESTEROL
EVEN-EVEN NUCLEI
GLUCOSE
HEXOSES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPIDS
MAMMALS
METABOLISM
MONOSACCHARIDES
NERVOUS SYSTEM
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RADIOISOTOPES
RATS
RODENTS
SACCHARIDES
STEROIDS
STEROLS
TRACER TECHNIQUES
VERTEBRATES
YEARS LIVING RADIOISOTOPES