Lipogenesis from U/sup 14/C lactate in obese Zucker rat hepatocytes. Effect of albumin-bound oleate

Journal Article · Mon Sep 10 04:00:00 EDT 1984 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(84)90193-0· OSTI ID:6047646

Porquet, D; Serbource-Goguel, N; Durand, G; Maccario, J; Feger, J; Agneray, J

Lipogenesis from U(/sup 14/C) lactate was studied in hepatocytes isolated from obese Zucker rats (fa/fa) their lean littermates (Fa/.) and Sprague Dawley rats. The distribution of radioactive carbon between the glycerol and the fatty acid moieties of the acylglycerols were studied. Radioactive lactate was better utilized for glycerol formation than it was for fatty acid formation in the obese rats. However, when oleate was added to the hepatocytic incubation medium, radioactive lactate was preferentially incorporated into the fatty acid moiety of the acyglycerols. Among the nutrients, lactate seems to be a better source of carbon than glucose for lipid synthesis. It has been shown that there is increased hepatic portal blood concentration of lactate several hours after eating: about 4 mM in Wistar rats and 10-15 mM in obese Zucher rats. We are interested in determin the incorporation of carbon from lactate either into glycerol or into fatty acid moieties of hepatic acylgylcerols, and in determining the influence of exogenous fatty acids on acylgylcerol synthesis, since a high level of circulating fatty acids in Zucher obese rats has been reported. The purpose was to determine the incorporaton of lactate into glycerol and fatty moieties of acylglycerols, under the influence of oleate.

• Research Organization: Universite Paris-Sud, France
• OSTI ID: 6047646
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 35:11; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
ANIMAL CELLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CHEMICAL BONDS
DIGESTIVE SYSTEM
ESTERS
GLANDS
GLYCEROL
HYDROXY ACIDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LACTIC ACID
LIPIDS
LIVER
MAMMALS
MONOCARBOXYLIC ACIDS
OLEIC ACID
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RADIOASSAY
RATS
REACTION KINETICS
RODENTS
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES