Effect of triiodothyronine and insulin on glucose metabolism in tissue explants and isolated adipocytes from lean and obese Zucker rats

Thesis/Dissertation · Mon Dec 31 23:00:00 EST 1984

OSTI ID:5219765

Bailey, J W

Glucose metabolism in adipocytes from 6 week old lean and obese Zucker rats were sensitive to direct and chronic treatment with insulin and triidothyronine (T/sub 3/). Insulin had a large stimulatory effect on glucose metabolism in acutely isolated adipocytes. This effect was greater in the lean than in the obese. Fatty acid, CO/sub 2/, and glycerol-glyceride formation from radiolabeled glucose was elevated in the obese over the leans. Pretreatment of isolated adipocytes with pharmacological concentrations of T/sub 3/ for 30 minutes prior to the measurement of glucose metabolism had a greater effect on lean than obese adipocytes. The presence of insulin was required to observe the acute effects of T/sub 3/. A 2-hour exposure to physiological levels of T/sub 3/ in the presence of insulin in both lean and obese adipocytes decreased lipogenesis. In the absence of insulin, a 2 hour pretreatment with physiological levels of T/sub 3/ in tissue from a euthyroid animal produced increased lipogenesis.

• Research Organization: Iowa State Univ. of Science and Technology, Ames (USA)
• OSTI ID: 5219765
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACUTE EXPOSURE
ALCOHOLS
ALDEHYDES
ANIMAL CELLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
CARBOHYDRATES
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBOXYLIC ACIDS
CHALCOGENIDES
CONNECTIVE TISSUE CELLS
DATA
ESTERS
EXPERIMENTAL DATA
FAT CELLS
GLUCOSE
GLYCEROL
HEXOSES
HORMONES
HYDROXY COMPOUNDS
INFORMATION
INSULIN
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIPIDS
MAMMALS
METABOLISM
MONOSACCHARIDES
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PEPTIDE HORMONES
RATS
REACTION KINETICS
RESPONSE MODIFYING FACTORS
RODENTS
SACCHARIDES
SOMATIC CELLS
THYROID HORMONES
TRACER TECHNIQUES
TRIGLYCERIDES
TRIIODOTHYRONINE
VERTEBRATES