Differential stability of c-myc mRNAS in a cell-free system

Journal Article · Fri Jul 01 04:00:00 EDT 1988 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.8.7.2860· OSTI ID:6470856

Pei, R; Calame, K

The authors have developed a simple cell-free system for studying the stability of different mRNAs in vitro. They demonstrate that the threefold greater stability in vivo of truncated c-myc mRNA (lacking exon 1) compared with that of full-length c-myc mRNA is maintained in our in vitro system. Chimeric mRNAs in which the first exon of c-myc was fused to immunoglobulin C ..cap alpha.. heavy chain of glyceraldehyde-3-phosphate dehydrogenase mRNAs were not rapidly degraded, demonstrating that c-myc exon 1 alone is not sufficient to tag mRNAs for rapid degradation. Competition experiments show that full-length c-myc mRNA is specifically recognized by a factor(s) responsible for its rapid degradation. This system will allow further characterization and purification of these factors.

• Research Organization: Dept. of Biological Chemistry and the Molecular Biology Institute, Univ. of California, Los Angeles, CA (US)
• OSTI ID: 6470856
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:7; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
CLONING
DATA
DNA HYBRIDIZATION
DNA-CLONING
ENZYMES
EXPERIMENTAL DATA
GENES
GLOBULINS
HYBRIDIZATION
IMMUNOGLOBULINS
IN VITRO
INFORMATION
MESSENGER-RNA
NUCLEIC ACIDS
NUCLEOTIDE DEHYDROGENASES
NUMERICAL DATA
ONCOGENES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PROTEINS
RNA
RNA PROCESSING
STABILITY