Structure and expression of the human L-myc gene reveal a complex pattern of alternative mRNA processing
Journal Article
·
· Mol. Cell. Biol.; (United States)
The authors' analyzed in detail the structure of the L-myc gene isolated from human placental DNA and characterized its expression in several small-cell lung cancer cell lines. The gene is composed of three exons and two introns spanning 6.6 kilobases in human DNA. Several distinct mRNA species are produced in all small-cell lung cancer cell lines that express L-myc. These transcripts are generated from a single gene by alternative splicing of introns 1 and 2 and by use of alternative polyadenylation signals. In some mRNAs that is a long open reading frame with a predicted translated protein of 364 residues. Amino acid sequence comparison with c-myc and N-myc demonstrated multiple discrete regions with extensive homology. In contrast, other mRNA transcripts, generated by alternative processing, could encode a truncated protein with a novel carboxy-terminal end.
- Research Organization:
- NCI-Navy Medical Oncology Branch, National Cancer Institute, Naval Hospital, and Uniformed Services Univ. of the Health Sciences, Bethesda, MD (US)
- OSTI ID:
- 6925540
- Journal Information:
- Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:1; ISSN MCEBD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200* -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMAL CELLS
ANIMALS
BODY
CARCINOMAS
CELL CULTURES
CLEAVAGE
CLONING
CRYSTAL STRUCTURE
DISEASES
DNA
DNA-CLONING
FETAL MEMBRANES
GENES
GENETIC MAPPING
LUNGS
MAMMALS
MAN
MAPPING
MEMBRANES
MESSENGER-RNA
MICROSTRUCTURE
MOLECULAR BIOLOGY
MOLECULAR STRUCTURE
NEOPLASMS
NUCLEIC ACIDS
NUCLEOPROTEINS
ONCOGENES
ORGANIC COMPOUNDS
ORGANS
PHENOTYPE
PLACENTA
PRIMATES
PROTEINS
RESPIRATORY SYSTEM
RNA
RNA PROCESSING
TUMOR CELLS
VERTEBRATES
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMAL CELLS
ANIMALS
BODY
CARCINOMAS
CELL CULTURES
CLEAVAGE
CLONING
CRYSTAL STRUCTURE
DISEASES
DNA
DNA-CLONING
FETAL MEMBRANES
GENES
GENETIC MAPPING
LUNGS
MAMMALS
MAN
MAPPING
MEMBRANES
MESSENGER-RNA
MICROSTRUCTURE
MOLECULAR BIOLOGY
MOLECULAR STRUCTURE
NEOPLASMS
NUCLEIC ACIDS
NUCLEOPROTEINS
ONCOGENES
ORGANIC COMPOUNDS
ORGANS
PHENOTYPE
PLACENTA
PRIMATES
PROTEINS
RESPIRATORY SYSTEM
RNA
RNA PROCESSING
TUMOR CELLS
VERTEBRATES