cDNA sequences of two apolipoproteins from lamprey
The messages for two small but abundant apolipoproteins found in lamprey blood plasma were cloned with the aid of oligonucleotide probes based on amino-terminal sequences. In both cases, numerous clones were identified in a lamprey liver cDNA library, consistent with the great abundance of these proteins in lamprey blood. One of the cDNAs (LAL1) has a coding region of 105 amino acids that corresponds to a 21-residue signal peptide, a putative 8-residue propeptide, and the 76-residue mature protein found in blood. The other cDNA (LAL2) codes for a total of 191 residues, the first 23 of which constitute a signal peptide. The two proteins, which occur in the high-density lipoprotein fraction of ultracentrifuged plasma, have amino acid compositions similar to those of apolipoproteins found in mammalian blood; computer analysis indicates that the sequences are largely helix-permissive. When the sequences were searched against an amino acid sequence data base, rat apolipoprotein IV was the best matching candidate in both cases. Although a reasonable alignment can be made with that sequence and LAL1, definitive assignment of the two lamprey proteins to typical mammalian classes cannot be made at this point.
- Research Organization:
- Univ. of California, San Diego, La Jolla
- OSTI ID:
- 6470422
- Journal Information:
- Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 26:6; ISSN BICHA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Deletion of the propeptide from human preproapolipoprotein A-II redirects cotranslation processing by signal peptidase
Human very-low-density lipoprotein receptor complementary DNA and deduced amino acid sequence and localization of its Gene (VLDLR) to chromosome band 9p24 by fluorescence in situ hybridization
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
APOLIPOPROTEINS
ATP
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGY
BLOOD
BLOOD PLASMA
BODY
BODY FLUIDS
COMPUTER CALCULATIONS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DNA
DNA SEQUENCING
ELECTROPHORESIS
GENETICS
GLANDS
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
LIPIDS
LIPOPROTEINS
LIVER
MATERIALS
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RECOMBINANT DNA
STRUCTURAL CHEMICAL ANALYSIS