Isolation of a cDNA clone encoding the amino-terminal region of human apolipoprotein B
Journal Article
·
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
A partial cDNA clone for the B-26 region of apolipoprotein B was isolated from an adult human liver DNA library by screening with an oligonucleotide probe derived from amino-terminal protein sequence obtained from purified B-26 peptide. Antisera against a synthetic 17-residue peptide whose amino acid sequence was encoded by the clone cross-reacts with apolipoproteins B-26, B-100, and B-48, but not with B-74. The nucleotide sequence immediately upstream from the amino terminus of B-26 codes for an apparent signal sequence, implying that the B-26 moiety is in an amino-terminal locus in the B-100 protein. That this sequence represents a 5' end region is further supported by primer extension analysis using a fragment of the cDNA clone and by S1 nuclease protection experiments using the corresponding region in a genomic clone.
- Research Organization:
- California Biotechnology, Inc., Mountain View
- OSTI ID:
- 5561619
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 83:5; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
Similar Records
cDNA sequences of two apolipoproteins from lamprey
Isolation and characterization of a cDNA clone encoding wheat germ agglutinin
cDNA cloning, sequence analysis, and chromosomal localization of the gene for human carnitine palmitoyltransferase
Journal Article
·
Mon Mar 23 23:00:00 EST 1987
· Biochemistry; (United States)
·
OSTI ID:6470422
Isolation and characterization of a cDNA clone encoding wheat germ agglutinin
Journal Article
·
Thu Oct 01 00:00:00 EDT 1987
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
·
OSTI ID:7000020
cDNA cloning, sequence analysis, and chromosomal localization of the gene for human carnitine palmitoyltransferase
Journal Article
·
Mon Jan 14 23:00:00 EST 1991
· Proceedings of the National Academy of Sciences of the United States of America; (United States)
·
OSTI ID:5079327
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
APOLIPOPROTEINS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
BODY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DNA
DNA SEQUENCING
GLANDS
ISOTOPES
LIGHT NUCLEI
LIPIDS
LIPOPROTEINS
LIVER
MAMMALS
MAN
MESSENGER-RNA
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
RECOMBINANT DNA
RNA
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS
TRANSCRIPTION
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
APOLIPOPROTEINS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
BODY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DNA
DNA SEQUENCING
GLANDS
ISOTOPES
LIGHT NUCLEI
LIPIDS
LIPOPROTEINS
LIVER
MAMMALS
MAN
MESSENGER-RNA
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
RECOMBINANT DNA
RNA
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS
TRANSCRIPTION
VERTEBRATES