Effects of ethanol on calcium transport across the liver cell plasma membrane

Journal Article · Wed Apr 01 04:00:00 EST 1987 · Res. Commun. Chem. Pathol. Pharmacol.; (United States)

OSTI ID:6470272

Bernstein, J; Santacana, G

The effect of ethanol on calcium transport by the liver cell was studied by using a rat liver slice preparation. Ethanol was shown to decrease by about 30% the rate constant for /sup 45/Ca efflux from the intracellular compartment. This inhibitory effect of ethanol was not observed in the absence of Ca/sup 2 +/ or Na/sup +/ from the incubation medium. Ethanol was also shown to greatly increase non-insulin calcium uptake by liver slices. This effect of ethanol appeared to be dose dependent and was not observed in the absence of Na/sup +/ from the incubation medium. The ability of ethanol to increase calcium uptake by the hepatocyte was completely blocked by 1 mM Amiloride. Amiloride, however, did not affect the increased entry of either Na/sup +/ or Ca/sup 2 +/ produced by 10 mM Ouabain, a specific inhibitor of the sodium pump. Carbon tetrachloride (CCl/sub 4/), a well known hepatotoxin, also increased calcium uptake by the hepatocyte. Amiloride, however, was not able to block the CCl/sub 4/-induced calcium uptake. These results suggest that ethanol activates a Na/sup +/ entry pathway, probably represented by a Na/sup +//H/sup +/ exchanger, which in turn stimulates an entry of Ca/sup 2 +/ through a Na/sup +//Ca/sup 2 +/ exchange mechanism located in the plasma membrane of the hepatocyte.

• Research Organization: Univ. of Puerto Rico School of Medicine, San Juan
• OSTI ID: 6470272
• Journal Information: Res. Commun. Chem. Pathol. Pharmacol.; (United States), Journal Name: Res. Commun. Chem. Pathol. Pharmacol.; (United States) Vol. 1; ISSN RCOCB
• Country of Publication: United States
• Language: English

Related Subjects

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560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ALKALI METAL COMPOUNDS
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
CALCIUM 45
CALCIUM COMPOUNDS
CALCIUM ISOTOPES
CARBOHYDRATES
CARBON TETRACHLORIDE
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CELL CONSTITUENTS
CELL MEMBRANES
CHLORINATED ALIPHATIC HYDROCARBONS
DAYS LIVING RADIOISOTOPES
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ETHANOL
EVEN-ODD NUCLEI
GLYCOSIDES
HALOGENATED ALIPHATIC HYDROCARBONS
HYDROXY COMPOUNDS
IN VITRO
INTERMEDIATE MASS NUCLEI
ION EXCHANGE
ISOTOPE APPLICATIONS
ISOTOPES
LIVER CELLS
MAMMALS
MEMBRANE TRANSPORT
MEMBRANES
NUCLEI
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OUABAIN
RADIOISOTOPES
RATS
RESPONSE MODIFYING FACTORS
RODENTS
SODIUM COMPOUNDS
SOMATIC CELLS
STROPHANTHINS
TRACER TECHNIQUES
VERTEBRATES