Cloning, sequence analysis, and expression of the large subunit of the human lymphocyte activation antigen 4F2
Among the earliest expressed antigens on the surface of activated human lymphocytes is the surface antigen 4F2. The authors have used DNA-mediated gene transfer and fluorescence-activated cell sorting to obtain cell lines that contain the gene encoding the large subunit of the human 4F2 antigen in a mouse L-cell background. Human DNAs cloned from these cell lines were subsequently used as hybridization probes to isolate a full-length cDNA clone expressing 4F2. Sequence analysis of the coding region has revealed an amino acid sequence of 529 residues. Hydrophobicity plotting has predicted a probable structure for the protein that includes an external carboxyl terminus, an internal leader sequence, a single hydrophobic transmembrane domain, and two possible membrane-associated domains. The 4F2 cDNA detects a single 1.8-kilobase mRNA in T-cell and B-cell lines. RNA gel blot analysis of RNA derived from quiescent and serum-stimulated Swiss 3T3 fibroblasts reveals a cell-cycle modulation of 4F2 gene expression: the mRNA is present in quiescent fibroblasts but increases 8-fold 24-36 hr after stimulation, at the time of maximal DNA synthesis.
- Research Organization:
- Yale Univ., New Haven, CT (United States)
- OSTI ID:
- 6469924
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:24
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANTIGENS
DNA-CLONING
CELL CYCLE
GENE REGULATION
LYMPHOCYTES
CHEMICAL ACTIVATION
CELL FLOW SYSTEMS
DNA SEQUENCING
FIBROBLASTS
HYBRIDIZATION
MAN
MESSENGER-RNA
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CLONING
CONNECTIVE TISSUE CELLS
DNA HYBRIDIZATION
LEUKOCYTES
MAMMALS
MATERIALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
RNA
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques