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Cloning, sequence analysis, and expression of the large subunit of the human lymphocyte activation antigen 4F2

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
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Among the earliest expressed antigens on the surface of activated human lymphocytes is the surface antigen 4F2. The authors have used DNA-mediated gene transfer and fluorescence-activated cell sorting to obtain cell lines that contain the gene encoding the large subunit of the human 4F2 antigen in a mouse L-cell background. Human DNAs cloned from these cell lines were subsequently used as hybridization probes to isolate a full-length cDNA clone expressing 4F2. Sequence analysis of the coding region has revealed an amino acid sequence of 529 residues. Hydrophobicity plotting has predicted a probable structure for the protein that includes an external carboxyl terminus, an internal leader sequence, a single hydrophobic transmembrane domain, and two possible membrane-associated domains. The 4F2 cDNA detects a single 1.8-kilobase mRNA in T-cell and B-cell lines. RNA gel blot analysis of RNA derived from quiescent and serum-stimulated Swiss 3T3 fibroblasts reveals a cell-cycle modulation of 4F2 gene expression: the mRNA is present in quiescent fibroblasts but increases 8-fold 24-36 hr after stimulation, at the time of maximal DNA synthesis.

Research Organization:
Yale Univ., New Haven, CT (USA)
OSTI ID:
6469924
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:24; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIGENS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CYCLE
CELL FLOW SYSTEMS
CHEMICAL ACTIVATION
CLONING
CONNECTIVE TISSUE CELLS
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
FIBROBLASTS
GENE REGULATION
HYBRIDIZATION
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MESSENGER-RNA
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
RNA
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES