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Influence of MHC on thymus repopulation following intrathymic transfer of mouse T-cell precursors

Journal Article · · Cell. Immunol.; (United States)
;
T-cell precursors (pre-T cells) traditionally have been detected by their ability to repopulate the thymus of heavily irradiated mice following intravenous injection. Recently, an assay system involving the direct injection of pre-T cells into the thymus of sublethally irradiated animals has been described. Here we report the results of experiments designed to evaluate the ability of bone marrow cells to produce thymic repopulation following intrathymic injection in a wide range of donor-host strain combinations. Irradiated (600 R) mice were injected intrathymically with 2 X 10(6) bone marrow cells which differed from the recipient with respect to their Thy-1 allotype and the percentage of thymus cells expressing either donor- or recipient-type Thy-1 was determined 9 to 23 days after injection. The results of these experiments showed that thymocytes expressing the Thy-1 allotype derived from the donor marrow were only detected when the donor and host were matched at MHC. By contrast, thymic repopulation by MHC-mismatched donor marrow cells could readily be observed when these cells were given intravenously.
Research Organization:
Louisiana State Univ. Medical Center, Shreveport
OSTI ID:
6467328
Journal Information:
Cell. Immunol.; (United States), Journal Name: Cell. Immunol.; (United States) Vol. 2; ISSN CLIMB
Country of Publication:
United States
Language:
English

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Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW CELLS
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
GRAFT-HOST REACTION
INJECTION
INTAKE
INTRAVENOUS INJECTION
IRRADIATION
LEUKOCYTES
LYMPHATIC SYSTEM
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
ORGANS
RADIATION EFFECTS
RODENTS
SOMATIC CELLS
SUBLETHAL IRRADIATION
THYMUS
VERTEBRATES