Influence of MHC on thymus repopulation following intrathymic transfer of mouse T-cell precursors

Journal Article · Wed Apr 01 00:00:00 EST 1987 · Cell. Immunol.; (United States)

DOI:https://doi.org/10.1016/0008-8749(87)90078-5· OSTI ID:6467328

Chervenak, R; Altazan, J D

T-cell precursors (pre-T cells) traditionally have been detected by their ability to repopulate the thymus of heavily irradiated mice following intravenous injection. Recently, an assay system involving the direct injection of pre-T cells into the thymus of sublethally irradiated animals has been described. Here we report the results of experiments designed to evaluate the ability of bone marrow cells to produce thymic repopulation following intrathymic injection in a wide range of donor-host strain combinations. Irradiated (600 R) mice were injected intrathymically with 2 X 10(6) bone marrow cells which differed from the recipient with respect to their Thy-1 allotype and the percentage of thymus cells expressing either donor- or recipient-type Thy-1 was determined 9 to 23 days after injection. The results of these experiments showed that thymocytes expressing the Thy-1 allotype derived from the donor marrow were only detected when the donor and host were matched at MHC. By contrast, thymic repopulation by MHC-mismatched donor marrow cells could readily be observed when these cells were given intravenously.

• Research Organization: Louisiana State Univ. Medical Center, Shreveport
• OSTI ID: 6467328
• Journal Information: Cell. Immunol.; (United States), Vol. 2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BONE MARROW CELLS
BIOLOGICAL RADIATION EFFECTS
GRAFT-HOST REACTION
LYMPHOCYTES
CELL PROLIFERATION
INTRAVENOUS INJECTION
MICE
SUBLETHAL IRRADIATION
THYMUS
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
INJECTION
INTAKE
IRRADIATION
LEUKOCYTES
LYMPHATIC SYSTEM
MAMMALS
MATERIALS
ORGANS
RADIATION EFFECTS
RODENTS
SOMATIC CELLS
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture