Thymic repopulation following intrathymic injection of mouse bone marrow cells in MHC matched and mismatched recipients
T cell precursors (pre-T cells) have traditionally been detected by their ability to repopulate the thymus of heavily irradiated mice following intravenous injection. Recently, Goldschneider et. al. developed an assay system which involves the direct injection of pre-T cells into the thymus. The authors used this technique to evaluate the ability of bone marrow cells to repopulate thymuses in various donor-host strain combinations. Sub-lethally irradiated (600R) mice were injected intrathymically with 2 x 10/sup 6/ bone marrow cells which differed from the recipient with respect to their Thy 1 allotype. The percentage of thymus cells expressing either the donor or recipient type Thy 1 marker was determined 14 to 21 days after injection. These experiments showed that in MHC matched donor-host combinations (AKR/cum ..-->.. AKR/J and CBA/J ..-->.. AKR/J), cells derived from the donor inoculum accounted for 40% to 75% of the total thymus population. MHC mismatched donor-host combinations (C57BL/6J ..-->.. AKR/J and Balb/c ..-->.. AKR/J) resulted in significantly less donor-type repopulation of the thymus. In these cases, donor repopulation typically ranged from 0% to 4%. The ability of the pre-T cells detected by intrathymic injection to proliferate in the thymic environment, therefore, appears to be influenced by the MHC. This may reflect commitment of pre-T cells to MHC haplotype recognition prior to their migration to the thymus.
- Research Organization:
- Louisiana State Univ. Medical Center, Shreveport
- OSTI ID:
- 5004380
- Report Number(s):
- CONF-8604222-; TRN: 86-037131
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
LYMPHOCYTES
CELL PROLIFERATION
THYMUS
IMMUNE REACTIONS
BONE MARROW CELLS
INJECTION
PRECURSOR
SUBLETHAL IRRADIATION
ANIMAL CELLS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
INTAKE
IRRADIATION
LEUKOCYTES
LYMPHATIC SYSTEM
MATERIALS
ORGANS
SOMATIC CELLS
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