Genetic dependence of recombination in recD mutants of Escherichia coli
RecBCD enzyme has multiple activities including helicase, exonuclease and endonuclease activities. Mutations in the genes recB or recC, encoding two subunits of the enzyme, reduce the frequency of many types of recombinational events. Mutations in recD, encoding the third subunit, do not reduce recombination even though most of the activities of the RecBCD enzyme are severely reduced. In this study, the genetic dependence of different types of recombination in recD mutants has been investigated. The effects of mutations in genes in the RecBCD pathway (recA and recC) as well as the genes specific for the RecF pathway (recF, recJ, recN, recO, recQ, ruv and lexA) were tested on conjugational, transductional and plasmid recombination, and on UV survival. recD mutants were hyper-recombinogenic for all the monitored recombination events, especially those involving plasmids, and all recombination events in recD strains required recA and recC. In addition, unlike recD+ strains, chromosomal recombination events and the repair of UV damage to DNA in recD strains were dependent on one RecF pathway gene, recJ. Only a subset of the tested recombination events were affected by ruv, recN, recQ, recO and lexA mutations.
- Research Organization:
- Dana-Farber Cancer Institute, Boston, MA (USA)
- OSTI ID:
- 6466494
- Journal Information:
- Genetics; (United States), Journal Name: Genetics; (United States) Vol. 120:1; ISSN GENTA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
BIOLOGICAL RADIATION EFFECTS
CELL CONSTITUENTS
DNA
DNA HELICASES
DNA-ASE
ELECTROMAGNETIC RADIATION
ENDONUCLEASES
ENZYME ACTIVITY
ENZYMES
ESCHERICHIA COLI
ESTERASES
GENE RECOMBINATION
GENES
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
GENOTYPE
HYDROLASES
MICROORGANISMS
MUTANTS
MUTATION FREQUENCY
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PLASMIDS
RADIATION EFFECTS
RADIATION INDUCED MUTANTS
RADIATIONS
ULTRAVIOLET RADIATION