Stimulation of granulocytic cell iodination by pine cone antitumor substances

Journal Article · Wed Feb 01 04:00:00 EST 1989 · J. Leukocyte Biol.; (United States)

OSTI ID:6465145

Unten, S; Sakagami, H; Konno, K

Antitumor substances (Fractions VI and VII) prepared from the NaOH extract of pine cone significantly stimulated the iodination (incorporation of radioactive iodine into an acid-insoluble fraction) of human peripheral blood adherent mononuclear cells, polymorphonuclear cells (PMN), and human promyelocytic leukemic HL-60 cells. In contrast, these fractions did not significantly increase the iodination of nonadherent mononuclear cells, red blood cells, other human leukemic cell lines (U-937, THP-1, K-562), human diploid fibroblast (UT20Lu), or mouse cell lines (L-929, J774.1). Iodination of HL-60 cells, which were induced to differentiate by treatment with either retinoic acid or tumor necrosis factor, were stimulated less than untreated cells. The stimulation of iodination of both PMN and HL-60 cells required the continuous presence of these fractions and was almost completely abolished by the presence of myeloperoxidase inhibitors. The stimulation activity of these fractions was generally higher than that of various other immunopotentiators. Possible mechanisms of extract stimulation of myeloperoxidase-containing cell iodination are discussed.

• Research Organization: Showa Univ., Tokyo (Japan)
• OSTI ID: 6465145
• Journal Information: J. Leukocyte Biol.; (United States), Journal Name: J. Leukocyte Biol.; (United States) Vol. 45:2; ISSN JLBIE
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANTINEOPLASTIC DRUGS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CELL DIFFERENTIATION
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
DRUGS
ENZYME INHIBITORS
FIBROBLASTS
GLYCOLYSIS
GROWTH FACTORS
HALOGENATION
IODINATION
IODINE ISOTOPES
ISOTOPES
LEUKOCYTES
MATERIALS
METABOLISM
MITOGENS
MONOCYTES
NEUTROPHILS
ORGANIC COMPOUNDS
PHAGOCYTOSIS
POLYSACCHARIDES
PROTEINS
SACCHARIDES
SOMATIC CELLS
TUMOR CELLS