Assay for activity of arogenate dehydratase based upon the selective oxidation of arogenate
Journal Article
·
· Anal. Biochem.; (United States)
An improved method of assay is presented for arogenate dehydratase, an enzyme catalyzing the formation of L-phenylalamine from L-arogenate. The improvement consists of the inclusion of a step in which arogenate is selectively oxidized using potassium permanganate prior to the measurement of phenylalanine. Following removal of excess KMnO/sub 4/, the phenylalanine present is measured fluorometrically under optimal conditions. A convenient qualitative test for the completeness of arogenate oxidation in the presence of other molecules which are oxidized by KMnO/sub 4/ is described. A rigorous evaluation of phenylalanine measurement by the method reported here was accomplished by comparison with three other methods, including amino acid analysis.
- Research Organization:
- State Univ. of New York, Binghamton
- OSTI ID:
- 6462856
- Journal Information:
- Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 110:1; ISSN ANBCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
AMINO ACIDS
BIOASSAY
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
CARBON-OXYGEN LYASES
CARBOXYLIC ACIDS
CATALYSTS
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
CHROMATOGRAPHY
COMPARATIVE EVALUATIONS
ENZYME ACTIVITY
ENZYMES
HYDRO-LYASES
KINETICS
LIQUID COLUMN CHROMATOGRAPHY
LYASES
MANGANESE COMPOUNDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDATION
OXYGEN COMPOUNDS
PERMANGANATES
PHENYLALANINE
POTASSIUM COMPOUNDS
POTASSIUM PERMANGANATES
QUANTITATIVE CHEMICAL ANALYSIS
QUANTITY RATIO
REACTION KINETICS
SAMPLING
SEPARATION PROCESSES
SYNTHESIS
TRANSITION ELEMENT COMPOUNDS
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
AMINO ACIDS
BIOASSAY
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
CARBON-OXYGEN LYASES
CARBOXYLIC ACIDS
CATALYSTS
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
CHROMATOGRAPHY
COMPARATIVE EVALUATIONS
ENZYME ACTIVITY
ENZYMES
HYDRO-LYASES
KINETICS
LIQUID COLUMN CHROMATOGRAPHY
LYASES
MANGANESE COMPOUNDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDATION
OXYGEN COMPOUNDS
PERMANGANATES
PHENYLALANINE
POTASSIUM COMPOUNDS
POTASSIUM PERMANGANATES
QUANTITATIVE CHEMICAL ANALYSIS
QUANTITY RATIO
REACTION KINETICS
SAMPLING
SEPARATION PROCESSES
SYNTHESIS
TRANSITION ELEMENT COMPOUNDS