Identification and characterization of the maize arogenate dehydrogenase gene family

Holding, David R.; Meeley, Robert B.; Hazebroek, Jan; Selinger, David; Gruis, Fred; Jung, Rudolf; Larkins, Brian A.

doi:10.1093/jxb/erq179

Identification and characterization of the maize arogenate dehydrogenase gene family

Journal Article · Thu Jun 17 00:00:00 EDT 2010 · Journal of Experimental Botany

DOI:https://doi.org/10.1093/jxb/erq179· OSTI ID:1625365

Holding, David R. ^[1]; Meeley, Robert B. ^[2]; Hazebroek, Jan ^[2]; Selinger, David ^[2]; Gruis, Fred ^[2]; Jung, Rudolf ^[2]; Larkins, Brian A. ^[3]

Univ. of Nebraska, Lincoln, NE (United States). Center for Plant Science Innovation; DOE/OSTI
Pioneer Hi-Bred International, Johnston, IA (United States)
Univ. of Arizona, Tucson, AZ (United States). Dept. of Plant Sciences

In plants, the amino acids tyrosine and phenylalanine are synthesized from arogenate by arogenate dehydrogenase and arogenate dehydratase, respectively, with the relative flux to each being tightly controlled. Here the characterization of a maize opaque endosperm mutant (mto140), which also shows retarded vegetative growth, is described The opaque phenotype co-segregates with a Mutator transposon insertion in an arogenate dehydrogenase gene (zmAroDH-1) and this led to the characterization of the four-member family of maize arogenate dehydrogenase genes (zmAroDH-1–zmAroDH-4) which share highly similar sequences. A Mutator insertion at an equivalent position in AroDH-3, the most closely related family member to AroDH-1, is also associated with opaque endosperm and stunted vegetative growth phenotypes. Overlapping but differential expression patterns as well as subtle mutant effects on the accumulation of tyrosine and phenylalanine in endosperm, embryo, and leaf tissues suggest that the functional redundancy of this gene family provides metabolic plasticity for the synthesis of these important amino acids. mto140/arodh-1 seeds shows a general reduction in zein storage protein accumulation and an elevated lysine phenotype typical of other opaque endosperm mutants, but it is distinct because it does not result from quantitative or qualitative defects in the accumulation of specific zeins but rather from a disruption in amino acid biosynthesis.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Arizona, Tucson, AZ (United States)

Sponsoring Organization:: National Science Foundation (NSF); Pioneer Hi-Bred International; USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: FG02-96ER20242

OSTI ID:: 1625365

Journal Information:: Journal of Experimental Botany, Journal Name: Journal of Experimental Botany Journal Issue: 13 Vol. 61; ISSN 0022-0957

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
arogenate dehydrogenase
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opaque mutant
plant sciences
tyrosine
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Identification and characterization of the maize arogenate dehydrogenase gene family

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