Methodologies for the separation of chemical classes from coal liquefaction materials

Conference · Thu Oct 31 23:00:00 EST 1985

OSTI ID:6461872

Wright, C W; Chess, E K; Lucke, R B; Weimer, W C; Later, D W; Stewart, D L; Mahlum, D D; Wilson, B W

Methods have been developed for the separation of the following chemical classes from complex coal liquefaction matrices by alumina column chromatography: aliphatic hydrocarbons, polycyclic aromatic hydrocarbons (PAH), nitrogen-containing polycyclic aromatic compounds (NPAC), and hydroxy-substituted PAH. Fractional distillation prior to column chromatography has been used as an additional means of reducing the chemical complexity of coal liquefaction materials. When the above fractions were tested for biological response in microbial mutagenicity and initiation/promotion mouse skin tumorigenicity assays, it was found that the majority of the mutagenicity was associated with the NPAC fractions. These two biologically active fractions were, therefore, further subfractionated. The NPAC fractions were separated into secondary amine (carbazoles), primary aromatic amine (amino-substituted PAH), tertiary amine (aza arene), and cyano-substituted PAH subfractions by column chromatography. The majority of the microbial mutagenicity was isolated in the amino-substituted PAH subfraction. The PAH (and hydroxy-PAH) fraction was subfractionated by molecular weight using reverse-phase high pressure liquid chromatography (HPLC). The majority of the tumorigenicity of the PAH fractions was not completely concentrated into any one HPLC subfraction. As coal liquefaction processes have evolved, the products have become more highly alkylated and hydrogenated. The hydroaromatic species in the coal liquefaction materials have been separated from the PAH species by picric-acid-coated alumina column chromatography. The hydroaromatic species were found to exhibit little to no mutagenic or tumorigenic response. 21 refs., 8 figs.

• Research Organization: Pacific Northwest Labs., Richland, WA (USA)
• DOE Contract Number: AC06-76RL01830
• OSTI ID: 6461872
• Report Number(s): PNL-SA-13284; CONF-8510225-5; ON: DE86004877
• Country of Publication: United States
• Language: English

Related Subjects

01 COAL, LIGNITE, AND PEAT
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Lignite
& Peat-- Hydrogenation & Liquefaction
010600 -- Coal
Lignite
& Peat-- Properties & Composition
016000* -- Coal
Lignite
& Peat-- Health & Safety
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMALS
AROMATICS
AZAARENES
BACTERIA
CARCINOGEN SCREENING
CHROMATOGRAPHY
COAL LIQUEFACTION
COAL LIQUIDS
DATA
DISTILLATES
DISTILLATION
EXPERIMENTAL DATA
EXXON LIQUEFACTION PROCESS
FLUIDS
FRACTIONATION
GAS CHROMATOGRAPHY
GEL PERMEATION CHROMATOGRAPHY
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
HYDROXY COMPOUNDS
INFORMATION
LIQUEFACTION
LIQUID COLUMN CHROMATOGRAPHY
LIQUIDS
MAMMALS
MASS SPECTROSCOPY
MICROORGANISMS
MUTAGEN SCREENING
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
POLYCYCLIC AROMATIC AMINES
POLYCYCLIC AROMATIC HYDROCARBONS
RATS
RODENTS
SALMONELLA
SALMONELLA TYPHIMURIUM
SCREENING
SEPARATION PROCESSES
SPECTROSCOPY
SRC PROCESS
THERMOCHEMICAL PROCESSES
TSL PROCESS
VERTEBRATES