Hydroxylamine as a competitive inhibitor for the kinetic enzymatic assay of ethanol in relatively concentrated solutions
Journal Article
·
· Biotechnol. Bioeng.; (United States)
The kinetic enzymatic assay of ethanol may be optimized for a selected range of substrate concentrations by a judicious choice of acetaldehyde trapping agent and ADH competitive inhibitor. Hydrazine and hydroxylamine can serve both of these functions. If the two agents were used simultaneously, hydrazine would serve as the more potent trapping agent and hydroxylamine as the more potent competitive inhibitor.
- Research Organization:
- Oak Ridge National Lab., TN
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 6455607
- Journal Information:
- Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 22; ISSN BIBIA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
AMINES
BIOASSAY
BIOCHEMICAL REACTION KINETICS
CHEMICAL PREPARATION
COENZYMES
ENZYME ACTIVITY
ENZYMES
ETHANOL
HYDRAZINE
HYDROXY COMPOUNDS
HYDROXYLAMINE
KINETICS
NAD
NITROGEN COMPOUNDS
NUCLEOTIDES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
REACTION KINETICS
REAGENTS
SUBSTRATES
SYNTHESIS
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
AMINES
BIOASSAY
BIOCHEMICAL REACTION KINETICS
CHEMICAL PREPARATION
COENZYMES
ENZYME ACTIVITY
ENZYMES
ETHANOL
HYDRAZINE
HYDROXY COMPOUNDS
HYDROXYLAMINE
KINETICS
NAD
NITROGEN COMPOUNDS
NUCLEOTIDES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
REACTION KINETICS
REAGENTS
SUBSTRATES
SYNTHESIS