An enzyme-amplified microtiter plate assay for ethanol: Its application to the detection of peanut ethanol and alcohol dehydrogenase

Conference · Fri Dec 01 04:00:00 EST 1995

OSTI ID:126039

Chung, S Y; Vercellotti, J R ^[1]; Sanders, T H ^[2]

---

1. USDA-ARS-SRRC, New Orleans, LA (United States)
2. USDA-ARS-NCSU, Raleigh, NC (United States)

A calorimetric microliter plate assay for ethanol amplified by aldehyde dehydrogenase (ALDH) was developed. In the assay ethanol from a sample took part in a chain-reaction catalyzed by alcohol dehydrogenase (ADH) and amplified by ALDH in the presence of NAD{sup +}, diaphorase, and p-ibdonitrotetrazolium-violet (INT-violet)(a precursor of red product). The resultant reaction gave a red color, the intensity of which was proportional to the amount of ethanol present. Using the technique, the content of activity from peanuts of differing maturity and curing stages were determined respectively. Data showed that immature peanuts had a higher level of ethanol and a lower ADH activity than mature peanuts, and that the level of ethanol and ADH activity decreased with the curing time. This indicates that peanut maturity and curing have an effect on ethanol. Also, this implies that other peanut volatiles could be affected in the same way as ethanol, a major volatile in peanuts.

• OSTI ID: 126039
• Report Number(s): CONF-950402--
• Country of Publication: United States
• Language: English

Related Subjects

40 CHEMISTRY
ALCOHOL DEHYDROGENASE
CHEMICAL COMPOSITION
ETHANOL
PEANUTS
QUALITATIVE CHEMICAL ANALYSIS