Determining the roles of the three alcohol dehydrogenases (AdhA, AdhB and AdhE) in Thermoanaerobacter ethanolicus during ethanol formation

Zhou, Jilai; Shao, Xiongjun; Olson, Daniel G.; Murphy, Sean Jean-Loup; Tian, Liang; Lynd, Lee R.

doi:10.1007/s10295-016-1896-6

Determining the roles of the three alcohol dehydrogenases (AdhA, AdhB and AdhE) in Thermoanaerobacter ethanolicus during ethanol formation

Journal Article · Mon May 01 00:00:00 EDT 2017 · Journal of Industrial Microbiology and Biotechnology

DOI:https://doi.org/10.1007/s10295-016-1896-6· OSTI ID:1924600

Zhou, Jilai ^[1]; Shao, Xiongjun ^[1]; Olson, Daniel G. ^[1]; Murphy, Sean Jean-Loup ^[1]; Tian, Liang ^[1]; Lynd, Lee R. ^[1]

0000 0001 2179 2404 grid.254880.3 Thayer School of Engineering Dartmouth College Hanover NH USA, grid.135519.a 0000 0004 0446 2659 Bioenergy Science Center Oak Ridge TN USA

Abstract

Thermoanaerobacter ethanolicus is a promising candidate for biofuel production due to the broad range of substrates it can utilize and its high ethanol yield compared to other thermophilic bacteria, such as Clostridium thermocellum. Three alcohol dehydrogenases, AdhA, AdhB and AdhE, play key roles in ethanol formation. To study their physiological roles during ethanol formation, we deleted them separately and in combination. Previously, it has been thought that both AdhB and AdhE were bifunctional alcohol dehydrogenases. Here we show that AdhE has primarily acetyl-CoA reduction activity (ALDH) and almost no acetaldehyde reduction (ADH) activity, whereas AdhB has no ALDH activity and but high ADH activity. We found that AdhA and AdhB have similar patterns of activity. Interestingly, although deletion of both adhA and adhB reduced ethanol production, a single deletion of either one actually increased ethanol yields by 60–70%.

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Sponsoring Organization:: USDOE

OSTI ID:: 1924600

Journal Information:: Journal of Industrial Microbiology and Biotechnology, Journal Name: Journal of Industrial Microbiology and Biotechnology Journal Issue: 4-5 Vol. 44; ISSN 1367-5435

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: Germany

Language:: English

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