Quantitation of mRNAs for α₁-acid glycoprotein and for serum albumin in livers of normal, stressed, fasted, and refed rats. [¹²⁵I or ¹³¹I radioimmunoassay for protein products of specific mRNA activity]

Thesis/Dissertation · Sun Jan 01 04:00:00 EST 1978

DOI:https://doi.org/10.2172/6453913· OSTI ID:6453913

Harris, Linda Jean ^[1]

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1. Univ. of Rochester, NY (United States)

A new procedure for determining the relative levels of a specific mRNA species was developed and applied to mRNA for rat serum albumin (RSA) and α₁-AGP) in rat liver. The method is a radioimmunoassay (¹²⁵In or ¹³¹I) for the completed protein, but which also detects antigenic determinants in nascent polypeptide chains on plysomes synthesizing the specific protein. Results show that 24 hs after stressing the rat by turpentine injection the total number of polysomes per mg DNA has increased by 20 to 25%; however, the number of RSA synthesizing polysomes per mg DNA has decreased slightly. In rats fasted for 6 days, the number of RSA synthesizing polysomes per mg polysomal RNA is only slightly below normal, but the total number of RSA synthesizing polysomes per mg DNA has decreased by 40%. Again, it is seen that RSA mRNA levels do not decrease as sharply as the rate of RSA synthesis. Twelve hours after refeeding the rats, the number of RSA synthesizing polysomes begins to increase, reaching a peak two to three times normal levels 24 to 48 hours after commencement of refeeding. During the first 24 hs after turpentine injection, there is a linear increase in the number of α₁-AGP synthesizing polysomes. The increase is smaller during the next 24 hs and there is a small decrease between 48 and 72 hs. The serum concentrations of α₁-AGP following turpentine treatment reflect these changes in polysome levels. It was not possible to compare the number of α₁-AGP synthesizing polysomes in livers of normal, fasted, and refed rats because the levels detected were only slightly higher than those seen in rat and rat kidney polysome controls. This background activity must be eliminated before the technique can be applied to quantitating mRNA for proteins synthesized in very small quantities. This technique offers several advantages over other procedures commonly used to quantitate mRNA. (ERB)

• Research Organization: Univ. of Rochester, NY (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: EY-76-C-02-3490
• OSTI ID: 6453913
• Report Number(s): UR--3490-1495
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ALBUMINS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL STRESS
BODY
CARBOHYDRATES
DATA
DATA FORMS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
EXPERIMENTAL DATA
FASTING
GLANDS
GLUCOPROTEINS
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE 131
IODINE ISOTOPES
ISOLATED VALUES
ISOTOPE APPLICATIONS
ISOTOPES
LIVER
MAMMALS
MEASURING METHODS
MESSENGER-RNA
NUCLEI
NUCLEIC ACIDS
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
QUANTITY RATIO
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
RATS
RNA
RODENTS
SACCHARIDES
TRACER TECHNIQUES
VERTEBRATES