The Entner-Doudoroff pathway in Escherichia coli is induced for oxidative glucose metabolism via pyrroloquinoline quinone-dependent glucose dehydrogenase

Journal Article · Mon Nov 30 23:00:00 EST 1992 · Applied and Environmental Microbiology; (United States)

OSTI ID:6448768

Fliege, R; Tong, Suxiang; Shibata, A; Nickerson, K W; Conway, T ^[1]

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1. Univ. of Nebraska, Lincoln (United States)

The Entner-Doudoroff pathway forms the core of central metabolism in many bacteria. However, the physiological role of the Entner-Doudoroff pathway in Escherichia coli is still unclear, and formal proof that oxidative glucose metabolism occurs via this pathway has not been reported. This paper provides direct evidence that the Entner-Doudoroff pathway is turned on by oxidation of glucose to gluconate in the periplasm. In addition the role of limiting phosphate in regulating the Entener-Doudoroff pathway is examined in this study, and it is concluded that, for E. coli, a low phosphate concentration promotes use of the Entner-Doudoroff pathway indirectly by providing access of pyrroloquinoline quinone (PQQ) into the periplasm rather than directly by derepressing edd and eda. Oxidative glucose metabolism, as opposed to phosphotransferase transport and glycolysis, may provide an advantage in aerobic, low phosphate, aquatic environments.

• DOE Contract Number: FG02-90ER20002
• OSTI ID: 6448768
• Journal Information: Applied and Environmental Microbiology; (United States), Journal Name: Applied and Environmental Microbiology; (United States) Vol. 58:12; ISSN AEMIDF; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AEROBIC CONDITIONS
ALDEHYDES
AQUATIC ECOSYSTEMS
AROMATICS
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
CARBOHYDRATES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
ECOSYSTEMS
ENZYMES
ESCHERICHIA COLI
GLUCONIC ACID
GLUCOSE
GLYCOLYSIS
HEXOSES
HYDROXY ACIDS
METABOLISM
MICROORGANISMS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
OXIDATION
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROTEINS
QUINONES
SACCHARIDES
TRANSFERASES
TRANSPORT