Nutritional complementation of oxidative glucose metabolism in Escherichia coli via pyrroloquinoline quinone-dependent glucose dehydrogenase and the Entner-Doudoroff pathway
- Univ. of Nebraska, Lincoln (USA)
Two glucose-negative Escherichia coli mutants (ZSC113 and DF214) were unable to grow on glucose as the sole carbon source unless supplemented with pyrroloquinoline quinone (PQQ). PQQ is the cofactor for the periplasmic enzyme glucose dehydrogenase, which converts glucose to gluconate. Aerobically, E. Coli ZSC113 grew on glucose plus PQQ with a generation time of 65 min, a generation time about the same as that for wild-type E. coli in a defined glucose-salts medium. Thus, for E. coli ZSC113 the Entner-Doudoroff pathway was fully able to replace the Embden-Meyerhof-Parnas pathway. In the presence of 5% sodium dodecyl sulfate, PQQ no longer acted as a growth factor. Sodium dodecyl sulfate inhibited the formation of gluconate from glucose but not gluconate metabolism. Adaptation to PQQ-dependent growth exhibited long lag periods, except under low-phosphate conditions, in which the PhoE porin would be expressed. The authors suggest that E. coli has maintained the apoenzyme for glucose dehydrogenase and the Entner-Doudoroff pathway as adaptations to an aerobic, low-phosphate, and low-detergent aquatic environment.
- DOE Contract Number:
- FG02-90ER20002
- OSTI ID:
- 5382625
- Journal Information:
- Applied and Environmental Microbiology; (United States), Journal Name: Applied and Environmental Microbiology; (United States) Vol. 57:7; ISSN 0099-2240; ISSN AEMID
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
540310* -- Environment
Aquatic-- Basic Studies-- (1990-)
AEROBIC CONDITIONS
ALDEHYDES
BACTERIA
BIOLOGICAL ADAPTATION
BIOLOGICAL PATHWAYS
CARBOHYDRATES
COENZYMES
ENVIRONMENT
ENZYMES
ESCHERICHIA COLI
GLUCOSE
HEXOSES
METABOLISM
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
SACCHARIDES