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Flow cytometric characterization of rat thymus cells in a radiation-dominated model of combined injury. Scientific report

Technical Report ·
OSTI ID:6441621
; ;
Thymuses of rats that had been: (a) gamma-irradiated 500 cGy whole-body radiation (R), or (b) thermally injured 20% BSA dorsal, scald burn (TI), or c) combined injured irradiation followed by burn (CI) were studied for involution and recovery processes after sublethal treatments. The expression of surface antigens on thymic cells before and after injuries was evaluated using the monoclonal antibodies and flow cytometric analysis. Thymic cellularity decreased to less than 1% of normal (N), age-matched rats by 4 days after R or CI. Recovery reached 60% to 70% of N by 28 days post treatments. Expression of OX7, OX8, W3/13, and W3/25 antigens all reached nadirs of 40% of N by day 4 after R and CI. Recovery of antigen expression, except for W3/25, was near completion by day 7 after R and CI. Changes in antigen expression after TI were less pronounced for all mcAB tested. Variations in relative labeling of nonlymphoid thymic cells with OX4 (Ia-antigen) reflected the disappearance and recovery of radiosensitive lymphoid thymocytes. The similarity of results after R and CI demonstrate that the model of CI is radiation-dominated. The addition of burn injury to radiation trauma had no synergistically damaging effect on the parameters studied.
Research Organization:
Armed Forces Radiobiology Research Inst., Bethesda, MD (USA)
OSTI ID:
6441621
Report Number(s):
AD-A-200644/3/XAB; AFRRI-SR-88-14
Country of Publication:
United States
Language:
English

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Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIBODIES
ANTIGENS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CELL FLOW SYSTEMS
INJURIES
IRRADIATION
LYMPHATIC SYSTEM
MAMMALS
ORGANS
RADIATION EFFECTS
RADIATION INJURIES
RADIOSENSITIVITY
RATS
RODENTS
SOMATIC CELLS
SURFACES
THYMOCYTES
THYMUS
THYMUS CELLS
VERTEBRATES