Rat monocytes in a model of combined injury express the OX8 antigen

Journal Article · Tue Sep 01 04:00:00 EDT 1987 · J. Leukocyte Biol.; (United States)

OSTI ID:6122196

Kaffenberger, W; Gruber, D F; MacVittie, T J

We have analyzed peripheral blood mononuclear cell preparations from a rat model of combined injury (CI) (whole-body irradiation (500 cGy /sup 60/Co) followed by a thermal injury (20% body surface area, dorsal, scald burn)) for the expression of OX8 antigens. Ficoll-separated mononuclear fractions were labeled with monoclonal antibodies MRC OX8, MRC OX19, W3/13 HLK, or W3/25 for flow cytometric analysis. Combined-injury trauma resulted in decreased mononuclear cells to 6% of normal. This effect was due to the rapid decrease in radiosensitive lymphocytes from 83% to 10%. The relative numbers of monocytes increased from a normal 13% to 70% at day 4 after CI. Labeling of cells with OX8 after CI shifted to a population which was significantly larger in volume than normal lymphocytes. At the same time the mean fluorescence intensity of OX8-positive cells was considerably reduced. With the use of a F(ab) fragment of OX8 as a probe, these results could be partially explained as unspecific binding of the whole molecule of OX8 to Fc receptors expressed by activated monocytes. But, double-labeling and cell-sorting experiments also revealed the expression of OX8 antigens by a subset of OX8+/OX19- monocytes after CI.

• Research Organization: Armed Forces Radiobiology Research Institute, Bethesda, MD
• OSTI ID: 6122196
• Journal Information: J. Leukocyte Biol.; (United States), Journal Name: J. Leukocyte Biol.; (United States) Vol. 42:3; ISSN JLBIE
• Country of Publication: United States
• Language: English

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