Ureide catabolism of soybeans

Journal Article · Sun Mar 01 04:00:00 EST 1987 · Plant Physiol.; (United States)

OSTI ID:6438419

Winkler, R G; Blevins, D G; Polacco, J C; Randall, D D

Allantoin catabolism studies have been extended to intact leaf tissue of soybean (Glycine max L. Merr.) Phenyl phosphordiamidate, one of the most potent urease inhibitors known does not inhibit /sup 14/CO/sub 2/ release from (2,7-/sup 14/C)allantoin (urea labeled), but inhibits urea dependent CO/sub 2/release greater than or equal to99.9% under similar conditions. Furthermore, /sup 14/CO/sub 2/ and /sup 14/C) allantoate are the only detectable products of (2,7-/sup 14/C)allantoin catabolism. Neither urea nor any other product were detected by analysis on HPLC organic acid or organic base columns although urea and all commercially available metabolites that have been implicated in allantoin and glyoxylate metabolism can be resolved by a combination of these two columns. In contrast, when allantoin was labeled in the two central, nonureido carbons ((4,5-/sup 14/C)allantoin), its catabolism to (/sup 14/)allantoate, /sup 14/CO/sub 2/,(/sup 14/C)glyoxylate, (/sup 14/C)glycine, and (/sup 14/C)serine in leaf discs could be detected. These data are fully consistent with the metabolism of allantoate by two amidohydrolase reactions (neither of which is urease) that occur at similar rates to release glyoxylate, which in turn is metabolized via the photorespiratory pathway. This is the first evidence that allantoate is metabolized without urease action to NH/sub 4//sup +/ and CO/sub 2/ and that carbons 4 and 5 enter the photorespiratory pathway.

• Research Organization: Univ. of Missouri, Columbia
• OSTI ID: 6438419
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 83:3; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALLANTOIN
AMIDES
AZOLES
BACTERIA
BIOLOGICAL PATHWAYS
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBONIC ACID DERIVATIVES
CATABOLISM
CHALCOGENIDES
CHEMICAL REACTIONS
CHROMATOGRAPHY
ENZYME INHIBITORS
GLYCINE HISPIDA
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEAVES
LEGUMINOSAE
LIQUID COLUMN CHROMATOGRAPHY
METABOLISM
METABOLITES
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PLANTS
RHIZOBIUM
SEPARATION PROCESSES
SYNTHESIS
TRACER TECHNIQUES
UREA