Metabolism of allantoin in soybeans
The metabolism of (2-7 /sup 14/C) and (4-5 /sup 14/C) allantoin has been studied in intact leaf tissue to elucidate the pathway of allantoin catabolism and its regulation. 1.3 mM (2-7 /sup 14/C) and 1.3 mM (4-5 /sup 14/C) DL allantoin release /sup 14/CO/sub 2/ from intact leaf discs at 0.9 and 0.08 ..mu..moles.hr/sup -1/.g fresh wt/sup -1/ respectively. The most potent urease inhibitor known, phenyl phosphordiamidate (PPD), inhibited urease in intact tissue at concentrations from 0.1 mM to 10 mM. In contrast /sup 14/CO/sub 2/ release from (2-7 /sup 14/C) and (4-5 /sup 14/C) allantoin was not inhibited by 1 mM PPD in 0.5 h and 1 h assays. These data are consistent with allantoate amido-hydrolase action. Intact tissue discs were incubated with (4-5 /sup 14/C) allantoin and analyzed for catabolites by ion exclusion HPLC. Allantoate, ureidoglycolate, and glyoxylate were identified by their retention time. Two /sup 14/C labelled peaks did not migrate with known standards. They are being characterized to identify their structure. These data are consistent with a pathway of catabolism including allantoate, ureidoglycolate and glyoxylate, NH/sub 3/ and CO/sub 2/.
- Research Organization:
- Univ. of Missouri, Columbia
- OSTI ID:
- 5384754
- Journal Information:
- Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 80:4; ISSN PLPHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ALLANTOIN
AMIDINASES
AZOLES
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
BODY
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CHALCOGENIDES
ENZYME INHIBITORS
ENZYMES
GLYCINE HISPIDA
HETEROCYCLIC COMPOUNDS
HYDROLASES
IMIDAZOLES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEAVES
LEGUMINOSAE
METABOLISM
METABOLITES
MICROORGANISMS
NON-PEPTIDE C-N HYDROLASES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PLANT TISSUES
PLANTS
RHIZOBIUM
TISSUES
TRACER TECHNIQUES
UREASE