Benzo(a)pyrene-binding proteins of hamster embryo cell nuclei: comparison of nuclear isolation procedures
Journal Article
·
· J. Cell. Biochem.; (United States)
Hamster embryo cells matabolize benzo(a)pyrene to derivatives that covalently modify nuclear macromolecules including proteins. Not all proteins are modified to the same extent nor by the same metabolites. In particular, a protein of apparent molecular weight 32,000 is highly modified by derivatives of trans-9,10-dihydro-9,10-dihydroxy B(a)P. This protein is shown here to be preferentially lost from nuclei during purification by centrifugation through high molarity sucrose solutions followed by osmotic shock. It does not appear to be a cytoplasmic contaminant, but shares many properties of an abundant protein from Xenopus laevis oocytes, nucleoplasmin.
- Research Organization:
- Oak Ridge National Lab., TN
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 6438062
- Journal Information:
- J. Cell. Biochem.; (United States), Journal Name: J. Cell. Biochem.; (United States) Vol. 18; ISSN JCEBD
- Country of Publication:
- United States
- Language:
- English
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OSTI ID:6893482
Related Subjects
550200* -- Biochemistry
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AROMATICS
BENZOPYRENE
CELL CONSTITUENTS
CELL NUCLEI
CHEMICAL BONDS
CONDENSED AROMATICS
ELECTROPHORESIS
HAMSTERS
HISTONES
HYDROCARBONS
MAMMALS
METABOLISM
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PROTEINS
PURIFICATION
RODENTS
VERTEBRATES
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AROMATICS
BENZOPYRENE
CELL CONSTITUENTS
CELL NUCLEI
CHEMICAL BONDS
CONDENSED AROMATICS
ELECTROPHORESIS
HAMSTERS
HISTONES
HYDROCARBONS
MAMMALS
METABOLISM
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PROTEINS
PURIFICATION
RODENTS
VERTEBRATES