Quantitative tracer studies of metabolic dynamics of animal cells growing in tissue culture
Journal Article
·
· Anal. Biochem.; (United States)
Metabolic dynamics of animal cells, growing in tissue culture, can be determined quantitatively by allowing the cells to metabolize radioactively labeled substrates under carefully controlled steady-state conditions. In order to avoid artifacts resulting from uncontrolled changes in physiological conditions, a steady-state apparatus for animal cells (SAFAC) has been constructed. In this device, cells in culture plates (max of 30) can be given radioactive substrate and incubated for various periods without disturbing the steady-state metabolism prior to killing. Subsequent analysis by two-dimensional paper chromatography and radioautography shows that metabolites are labeled rapidly and thereafter are maintained at constant levels of radioactivity, as expected for steady-state metabolism.
- Research Organization:
- Univ. of California, Berkeley
- OSTI ID:
- 6438041
- Journal Information:
- Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 61; ISSN ANBCA
- Country of Publication:
- United States
- Language:
- English
Similar Records
INVESTIGATION OF THE METABOLISM OF TISSUE IN CULTURE WITH RADIOACTIVE TRACERS
I. Lipid metabolism stimulated by altered intracellular calcium in cultured fibroblasts. II. Regulation of the activity of rat adipose tissue lipoprotein lipase
THE USE OF THE TRACER TECHNIQUE IN THE INVESTIGATIONS OF THE HORMONAL EFFECT ON BONE TISSUE METABOLISM
Technical Report
·
Fri Oct 30 23:00:00 EST 1959
·
OSTI ID:4292788
I. Lipid metabolism stimulated by altered intracellular calcium in cultured fibroblasts. II. Regulation of the activity of rat adipose tissue lipoprotein lipase
Thesis/Dissertation
·
Thu Dec 31 23:00:00 EST 1987
·
OSTI ID:5159637
THE USE OF THE TRACER TECHNIQUE IN THE INVESTIGATIONS OF THE HORMONAL EFFECT ON BONE TISSUE METABOLISM
Technical Report
·
Fri Oct 30 23:00:00 EST 1959
·
OSTI ID:4270325