I. Lipid metabolism stimulated by altered intracellular calcium in cultured fibroblasts. II. Regulation of the activity of rat adipose tissue lipoprotein lipase
Thesis/Dissertation
·
OSTI ID:5159637
The cell killing process of 3T3 Swiss mouse fibroblasts stimulated by Ca{sup 2+} plus A23187, a Ca{sup 2+} ionophore has been studied. The aim of this research is to understand the biochemical mechanism of this process, i.e, to elucidate the step involved and to characterize the enzymes involved with each steps in the lipid metabolism stimulated in cultured fibroblasts undergoing a toxic death response. Parallel 3T3 cultures biosynthetically labeled with lipid precursors were examined under Ca{sup 2+}-mediated killing conditions. Labeled lipids were extracted and analyzed by thin-layer chromatography and autoradiography. Evidence for activation of a phosphatidylinositol-specific phospholipase C has been obtained in injured 3T3 cells labeled with ({sup 3}H)glycerol and ({sup 3}H)inositol. To simplify the system for studying the lipoprotein lipase reaction, our laboratory prepared the chromophore containing a substrate: 1,2-dipalmitoyl-3-{beta}-2-furylacryloyltriacylglycerol (DPFATG). By using this artificial lipid we could readily investigate the lipoprotein lipase reactions, since the absorbance change directly represents the hydrolysis of the chromophoric side chain of the substrate.
- Research Organization:
- Minnesota Univ., Minneapolis, MN (USA)
- OSTI ID:
- 5159637
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADIPOSE TISSUE
ALCOHOLS
ALKALINE EARTH METAL COMPOUNDS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AUTORADIOGRAPHY
BIOLOGICAL FUNCTIONS
BODY
CALCIUM COMPOUNDS
CELL KILLING
CHROMATOGRAPHY
CONNECTIVE TISSUE
CONNECTIVE TISSUE CELLS
ENZYME ACTIVITY
ESTERS
FIBROBLASTS
FUNCTIONS
GLYCEROL
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
LIPASE
LIPIDS
MAMMALS
METABOLISM
MICE
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
RATS
RODENTS
SEPARATION PROCESSES
SOMATIC CELLS
THIN-LAYER CHROMATOGRAPHY
TISSUES
TRITIUM COMPOUNDS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ADIPOSE TISSUE
ALCOHOLS
ALKALINE EARTH METAL COMPOUNDS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AUTORADIOGRAPHY
BIOLOGICAL FUNCTIONS
BODY
CALCIUM COMPOUNDS
CELL KILLING
CHROMATOGRAPHY
CONNECTIVE TISSUE
CONNECTIVE TISSUE CELLS
ENZYME ACTIVITY
ESTERS
FIBROBLASTS
FUNCTIONS
GLYCEROL
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
LIPASE
LIPIDS
MAMMALS
METABOLISM
MICE
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
RATS
RODENTS
SEPARATION PROCESSES
SOMATIC CELLS
THIN-LAYER CHROMATOGRAPHY
TISSUES
TRITIUM COMPOUNDS
VERTEBRATES