Characterization of some isolates of newly recovered avian sarcoma virus. [X Radiation]

Journal Article · Mon Jan 01 00:00:00 EST 1979 · J. Virol.; (United States)

OSTI ID:6433138

Halpern, C C; Hayward, W S; Hanafusa, H

We previously reported the isolation of a newly recovered avian sarcoma virus (rASV) from tumors of chickens injected with transformation-defective (td) mutants of the Schmidt--Ruppin strain of Rous sarcoma virus (SR--RSV). In this paper, we present further biological and biochemical characterization of the recovered sarcoma viruses. High titers of rASV's were generally obtained by cocultivation of tumor cells with normal chicken embryo fibroblasts or by homogenization of tumor tissues. Most rASV isolates were similar to SR--RSV, subgroup A (SR--RSV--A), in their growth characteristics and were nondefective in replication. The subgroup specificity of rASV's and the electrophoretic mobilities of their structural proteins were the same as those of parental td viruses. The nondefectiveness of rASV's was further substantiated by the size of their genomic RNA, which was indistinguishable from that of SR--RSV--AA and substantially larger than that of parental td RNA. Molecular hybridization using complementary DNA specific to the src gene of SR--RSV (cDNA/sub src/) showed that the RNAs of td mutants used in this study contained extensive deletions within the src gene (7 to 30% hybridization with cDNA/sub src/); the same probe hybridized up to 90% with RNA from two isolates of rASV. These data indicate that rASV has regained genetic information which had been deleted in the td mutants and strongly suggest that the generation of rASV involves a genetic interaction between td virus and host cell genetic information.

• Research Organization: Rockefeller Univ., New York, NY
• OSTI ID: 6433138
• Journal Information: J. Virol.; (United States), Vol. 29:1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
ONCOGENIC VIRUSES
BIOCHEMISTRY
BIOLOGICAL RADIATION EFFECTS
GENETICS
CHICKENS
DNA
FIBROBLASTS
HYBRIDIZATION
MUTANTS
NEOPLASMS
RNA
TUMOR CELLS
X RADIATION
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGY
BIRDS
CHEMISTRY
CONNECTIVE TISSUE CELLS
DISEASES
ELECTROMAGNETIC RADIATION
FOWL
IONIZING RADIATIONS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
RADIATION EFFECTS
RADIATIONS
SOMATIC CELLS
VERTEBRATES
VIRUSES
560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)
550200 - Biochemistry
550400 - Genetics