Effects of X-irradiation and sodium butyrate on cell-cycle traverse on normal and radiosensitive lymphoblastoid cells

Journal Article · Tue Oct 01 00:00:00 EDT 1985 · Exp. Cell Res.; (United States)

DOI:https://doi.org/10.1016/0014-4827(85)90180-6· OSTI ID:6429034

Smith, P J; Anderson, C O; Watson, J V

We have used a multi-parameter flow-cytometric technique to analyse changes in cell-cycle phase distribution (early and late G1, S and G2+M phases) for normal and X-ray-sensitive (ataxia-telangiectasia, A-T) lymphoblastoid cells exposed to X-irradiation and sodium butyrate (either alone or in combination). Sodium butyrate, an inhibitor of histone deacetylase, is a useful pharmacological tool for determining the proposed role of a histone acetylation-based chromatin surveillance system in controlling cell-cycle responses to DNA damage. We report that X-irradiated A-T cells (acute doses up to 1.5 Gy) demonstrate deficiencies in the capacity to traverse G1 and G2+M phases, although we can find no evidence of the specific involvement of a sodium butyrate-sensitive process in normal cells or abnormalities in the responses of A-T cells to the drug. We conclude that abnormal cellular control of G1 transition in A-T may be the basis of disturbed cellular differentiation in vivo, particularly in non-proliferating tissues under conditions of accumulated environmental or spontaneous DNA damage.

• Research Organization: MRC Clinical Oncology and Radiotherapeutics Unit, Cambridge, England
• OSTI ID: 6429034
• Journal Information: Exp. Cell Res.; (United States), Vol. 2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BUTYRIC ACID
GENETIC EFFECTS
LYMPHOCYTES
GENETIC RADIATION EFFECTS
CELL CYCLE
CHROMATIN
DNA
ENZYME INHIBITORS
RADIOSENSITIVITY
X RADIATION
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
LEUKOCYTES
MATERIALS
MONOCARBOXYLIC ACIDS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
SOMATIC CELLS
560121* - Radiation Effects on Cells- External Source- (-1987)