Effects of X-irradiation and sodium butyrate on cell-cycle traverse on normal and radiosensitive lymphoblastoid cells

Journal Article · Tue Oct 01 04:00:00 EDT 1985 · Exp. Cell Res.; (United States)

DOI:https://doi.org/10.1016/0014-4827(85)90180-6· OSTI ID:6429034

Smith, P J; Anderson, C O; Watson, J V

We have used a multi-parameter flow-cytometric technique to analyse changes in cell-cycle phase distribution (early and late G1, S and G2+M phases) for normal and X-ray-sensitive (ataxia-telangiectasia, A-T) lymphoblastoid cells exposed to X-irradiation and sodium butyrate (either alone or in combination). Sodium butyrate, an inhibitor of histone deacetylase, is a useful pharmacological tool for determining the proposed role of a histone acetylation-based chromatin surveillance system in controlling cell-cycle responses to DNA damage. We report that X-irradiated A-T cells (acute doses up to 1.5 Gy) demonstrate deficiencies in the capacity to traverse G1 and G2+M phases, although we can find no evidence of the specific involvement of a sodium butyrate-sensitive process in normal cells or abnormalities in the responses of A-T cells to the drug. We conclude that abnormal cellular control of G1 transition in A-T may be the basis of disturbed cellular differentiation in vivo, particularly in non-proliferating tissues under conditions of accumulated environmental or spontaneous DNA damage.

• Research Organization: MRC Clinical Oncology and Radiotherapeutics Unit, Cambridge, England
• OSTI ID: 6429034
• Journal Information: Exp. Cell Res.; (United States), Journal Name: Exp. Cell Res.; (United States) Vol. 2; ISSN ECREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
BUTYRIC ACID
CARBOXYLIC ACIDS
CELL CYCLE
CHROMATIN
CONNECTIVE TISSUE CELLS
DNA
ELECTROMAGNETIC RADIATION
ENZYME INHIBITORS
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
IONIZING RADIATIONS
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MONOCARBOXYLIC ACIDS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
SOMATIC CELLS
X RADIATION