Inhibitory effect of iron on the uptake of lead by erythrocytes

Journal Article · Tue Dec 31 23:00:00 EST 1974 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(75)90072-7· OSTI ID:6428292

Kaplan, M; Jones, A G; Davis, M A; Kopito, L

It is well known that more than 90% of the lead found in blood is associated with the erythrocytes. The present in vitro experiments show that the uptake of lead-203 by rabbit erythrocytes is inhibited by the presence of non-radioactive lead or iron or by reduction of the incubation temperature. The inhibitory effect of iron on radioactive lead uptake by erythrocytes is also demonstrable in vivo. When lead-203 is incorporated into erythrocytes in vitro, about 10% of the radioactivity is attached to the membrane and the remainder is found in the cytoplasm associated with hemoglobin and an unidentified low molecular weight intracellular component. In the presence of 25 ..mu..g/ml of added iron (Fe/sup + + +/) the uptake of radioactive lead by erythrocytes is reduced to 21.7+/-5.1% and membrane binding accounts for approximately 5% of this total. Chromatographic analyses of hemolysates show that the reduction in cytoplasmic labeling is directly related to decreased lead binding to the low molecular weight component, since hemoglobin binding remains unchanged. This work suggests that in addition to the interaction between iron and lead which occurs during the biosynthesis of heme, these metals may directly compete for specific erythrocyte binding sites. 22 references, 2 figures, 2 tables.

• Research Organization: Harvard School of Dental Medicine, Boston, MA
• OSTI ID: 6428292
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 16:10; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

560172* -- Radiation Effects-- Nuclide Kinetics & Toxicology-- Animals-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHROMATOGRAPHY
DATA
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
ERYTHROCYTES
EVEN-ODD NUCLEI
EXPERIMENTAL DATA
GLOBIN
HEAVY NUCLEI
HEME
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IN VITRO
IN VIVO
INFORMATION
IRON
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LEAD
LEAD 203
LEAD ISOTOPES
MAMMALS
MATERIALS
MEMBRANES
METALS
MOLECULAR WEIGHT
NUCLEI
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
RABBITS
RADIOISOTOPES
RADIONUCLIDE KINETICS
SECONDS LIVING RADIOISOTOPES
SEPARATION PROCESSES
TEMPERATURE DEPENDENCE
TRANSITION ELEMENTS
UPTAKE
VERTEBRATES