Enzymatic determination of carbon-14 labeled L-alanine in biological samples

Journal Article · Wed Jul 15 04:00:00 EDT 1987 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00141a022· OSTI ID:6426178

Serra, F; Palou, A; Pons, A

A method for determination of L-alanine-specific radioactivity in biological samples is presented. This method is based on the specific enzymatic transformation of L-alanine to pyruvic acid hydrazone catalyzed by the enzyme L-alanine dehydrogenase, formation of the pyruvic acid 2,4-dinitrophenylhydrazone derivative, and quantitative trapping in Amberlite XAD-7 columns, followed by radioactivity counting of the lipophilic eluate. No interferences from other UC-labeled materials such as D-glucose, glycerol, L-lactate, L-serine, L-glutamate, L-phenylalanine, glycine, L-leucine, and L-arginine were observed. This inexpensive and high-speed method is applicable to the simultaneous determination of L-alanine-specific radioactivity for a large number of samples.

• Research Organization: Universitat de Les Illes Balears, Spain
• OSTI ID: 6426178
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 59:14; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400101 -- Activation
Nuclear Reaction
Radiometric & Radiochemical Procedures
400102* -- Chemical & Spectral Procedures
ACTIVITY LEVELS
ALANINE-ALPHA
ALANINE-L
ALANINES
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
CARBON 14
CARBON ISOTOPES
CARBOXYLIC ACIDS
CHEMICAL ANALYSIS
CHEMICAL REACTION YIELD
CHEMICAL REACTIONS
DATA
ENZYMES
EVEN-EVEN NUCLEI
EXPERIMENTAL DATA
INFORMATION
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MATERIALS
NUCLEI
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
QUANTITATIVE CHEMICAL ANALYSIS
RADIOISOTOPES
YEARS LIVING RADIOISOTOPES
YIELDS