Polysaccharides from heterocyst and spore envelopes of a blue-green alga. [Anabaena cylindrica]

Journal Article · Sat Feb 10 04:00:00 EST 1979 · J. Biol. Chem.; (United States)

OSTI ID:6425915

Cardemil, L ^[1]; Wolk, C P

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1. Universidad de Chile, Santiago

The polysaccharides from the envelopes of heterocysts and spores of Anabaena cylindrica consist of repeating units containing 1 mannosyl and 3 glucosyl residues, all linked by ..beta..(1 ..-->.. 3) glucosidic bonds, with glucose, xylose, galactose, and mannose present in side branches. Degradation of the polysaccharides with specific glycosidases has permitted identification of the linkages to almost all of the branches. When the polysaccharides, from which all but two types of side branches had been cleaved, were digested with a ..beta..(1 ..-->.. 3) endoglucanase, glucose, a tri-, and a pentasaccharide were produced. The oligosaccharide products were identified. The backbones of the polysaccharides were sequenced from the reducing terminus by a modified Smith degradation. Analysis with NaB/sup 3/H/sub 4/ at each stage of the degradation showed that the backbones terminate in the sequence Man-Glc-Glc-Glc and are therefore presumed to have the structure (Man-Glc-Glc-Glc)/sub n/, and that they contain an average of from 128 to 150 sugar residues. From the information obtained, the repeating sequences of the original polysaccharides from the two types of differentiated cells of A. cylindrica could be largely deduced and appeared to be identical.

• OSTI ID: 6425915
• Journal Information: J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 254:3; ISSN JBCHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ALGAE
BIOCHEMISTRY
CARBOHYDRATES
CELL CONSTITUENTS
CELL WALL
CHEMISTRY
DATA
DATA FORMS
EXPERIMENTAL DATA
INFORMATION
ISOLATED VALUES
MOLECULAR STRUCTURE
NUMERICAL DATA
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SPORES