Comparative amperometric study of uptake hydrogenase and hydrogen photoproduction activities between heterocystous cyanobacterium Anabaena cylindrica B629 and nonheterocystous cyanobacterium Oscillatoria sp. strain Miami BG7

Journal Article · Thu Aug 01 04:00:00 EDT 1985 · Appl. Environ. Microbiol.; (United States)

OSTI ID:5918728

Kumazawa, S; Mitsui, A

Heterocystous filamentous cyanobacterium Anabaena cylindrica B629 and nonheterocystous filamentous cyanobacterium Oscillatoria sp. strain Miami BG7 were cultured in media with N/sub 2/ as the sole nitrogen source; and activities of oxygen-dependent hydrogen uptake, photohydrogen production photooxygen evolution, and respiration were compared amperometrically under the same or similar experimental conditions for both strains. Distinct differences in these activities were observed in both strains. The rates of hydrogen photoproduction and hydrogen accumulation were significantly higher in Oscillatoria sp. strain BG7 than in A. cylindrica B629 at every light intensity tested. The major reason for the difference was attributable to the fact that the heterocystous cyanobacterium had a high rate of oxygen-dependent hydrogen consumption activity and the nonheterocystous cyanobacterium did not. The activity of oxygen photoevolution and respiration also contributed to the difference. Oscillatoria sp. strain BG7 had lower O/sub 2/ evolution and higher respiration than did A. cylindrica B629. Thus, the effect of O/sub 2/ on hydrogen photoproduction was minimized in Oscillatoria sp. strain BG7. 32 references, 5 figures.

• Research Organization: Univ. of Miami, FL
• OSTI ID: 5918728
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 50:2; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

08 HYDROGEN
080106* -- Hydrogen-- Production-- Biosynthesis & Photochemical Processes
BIOLOGICAL VARIABILITY
CHEMICAL REACTIONS
COMPARATIVE EVALUATIONS
CULTURE MEDIA
CYANOBACTERIA
DATA
ELEMENTS
ENZYME ACTIVITY
ENZYMES
EXPERIMENTAL DATA
HYDROGEN
HYDROGENASES
INFORMATION
MICROORGANISMS
NITROGEN
NONMETALS
NUMERICAL DATA
OXIDOREDUCTASES
OXYGEN
PHOTOCHEMICAL REACTIONS
RESPIRATION