Phosphorylated tyrosine in the flagellum filament protein of Pseudomonas aeruginosa
Journal Article
·
· Journal of Bacteriology; (USA)
OSTI ID:6422065
- Univ. of Tennessee, Knoxville (USA)
Purified flagella from two strains of {sup 32}P-labeled Pseudomonas aeruginosa were shown to be phosphorylated. This was confirmed by autoradiography of flagellin protein in polyacrylamide gels. Thin-layer electrophoresis and autoradiography of flagellin partial hydrolysates indicated that phosphotyrosine was the major phosphorylated amino acid. High-pressure liquid chromatographic analysis confirmed the presence of phosphotyrosine in flagellum filament protein. Preliminary data indicated that less than one tyrosine per subunit was phosphorylated. No evidence was found for phosphorylation of serine or threonine. A function related to tyrosine phosphorylation has not been determined.
- OSTI ID:
- 6422065
- Journal Information:
- Journal of Bacteriology; (USA), Journal Name: Journal of Bacteriology; (USA) Vol. 172:9; ISSN JOBAA; ISSN 0021-9193
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550501* -- Metabolism-- Tracer Techniques
550701 -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AUTORADIOGRAPHY
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
ELECTROPHORESIS
HYDROXY ACIDS
ISOTOPES
LIGHT NUCLEI
LIQUID COLUMN CHROMATOGRAPHY
MICROORGANISMS
MOLECULAR WEIGHT
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORYLATION
PSEUDOMONAS
RADIOISOTOPES
SEPARATION PROCESSES
TYROSINE
550701 -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AUTORADIOGRAPHY
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
ELECTROPHORESIS
HYDROXY ACIDS
ISOTOPES
LIGHT NUCLEI
LIQUID COLUMN CHROMATOGRAPHY
MICROORGANISMS
MOLECULAR WEIGHT
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORYLATION
PSEUDOMONAS
RADIOISOTOPES
SEPARATION PROCESSES
TYROSINE