Immunoradiometric assay of endotoxin in serum. [/sup 125/I tracer technique, rats, Escherichia coli, Salmonella]

Journal Article · Mon Jan 01 04:00:00 EST 1979 · Clin. Chem.; (United States)

OSTI ID:6421475

Leibowitz, A I; Vladutiu, A O; Nolan, J P

We described an immunoradiometric assay for the specific detection and quantitation of endotoxin from Escherichia coli 026, with use of /sup 125/I-labeled antibody. The sensitivity of this assay is 1 ..mu..g/L. E. coli 026 endotoxin was detected in serum of rats for as long as 24 h after intraperitoneal injection of 0.1 mg of endotoxin per 100 g body weight. The assay is specific for the O-polysaccharide portion of this endotoxin, as confirmed by results after protein digestion and oxidation of the endotoxin, as well as by failure to detect endotoxin in serum after similar injections of E. coli 0127 and Salmonella typhosa 0901 endotoxins.

• Research Organization: State Univ. of New York, Buffalo
• OSTI ID: 6421475
• Journal Information: Clin. Chem.; (United States), Journal Name: Clin. Chem.; (United States) Vol. 25:1; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIGENS
BACTERIA
BETA DECAY RADIOISOTOPES
BLOOD SERUM
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENDOTOXINS
ESCHERICHIA COLI
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
RATS
RODENTS
SALMONELLA
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
VERTEBRATES