Investigation of separation mechanisms in the normal and reversed phase chromatography of polynuclear aromatic hydrocarbons on an amino bonded phase column. [n-propyl amine]

Technical Report · Tue Jan 01 04:00:00 EST 1980

OSTI ID:6417349

Karlesky, D; Shelly, D C; Warner, I

Experiments were run using an n-propyl amine polar bonded phase (Chromosorb LC-9) liquid chromatographic column in both the normal and reversed phase mode. Results indicate that the mechanism of separation in the normal phase is due mainly to a charge transfer interaction between the lone pair electrons on the stationary phase nitrogen and the ..pi.. electron cloud of the solute PNAs. Elution order seems to depend upon a combination of ..pi.. energy, electron affinity, and type of ring condensation of the solute. Comparing plots of log I versus number of aromatic carbons for catacondensed PNAs suggest that while the specific interaction is different than that seen in silica chromatography, the overall adsorption effect is comparable. In the reversed phase the separation mechanism is either a pure partitioning effect in highly polar mobile phases (methanol/water), or a mixture of liquid-solid adsorption and liquid-liquid partition in less polar solvent systems (acetonitrile/water).

• Research Organization: Texas A and M Univ., College Station (USA). Dept. of Chemistry
• DOE Contract Number: AS05-80EV10404
• OSTI ID: 6417349
• Report Number(s): DOE/EV/10404-6; ON: DE81028461
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400105* -- Separation Procedures
ADSORPTION
ALCOHOLS
AMINES
AROMATICS
CHROMATOGRAPHY
COLUMN PACKING
ELUTRIATION
HYDROCARBONS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
KINETICS
LIQUID COLUMN CHROMATOGRAPHY
MATERIALS
METHANOL
NITRILES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PACKINGS
POLAR COMPOUNDS
POLYCYCLIC AROMATIC HYDROCARBONS
REACTION KINETICS
SEPARATION PROCESSES
SORPTION
WATER