Limitations of the NNS assay for reducing sugars from saccharified lignocellulosics. [Trichoderma reesei]
Journal Article
·
· Biotechnol. Bioeng.; (United States)
An evaluation is presented of two DNS (2,4-dinitrosalicylic acid) assay procedures as well as high-performance liquid chromatography (HPLC) and YSI Glucose Analyzer analyses of sugars resulting from enzymatic saccharification of lignocellulosics. Trichoderma reesei was used to produce the cellulase system containing endoglucanase, cellobiohydrolase and cellobiase. Data suggest that the DNS assay can be used as an accurate analytical method for the evaluation of reducing sugars in pure solution as well as in supernatants from enzymatic saccharification if glucose is the sole product. However, only specific assay methods such as HPLC and YSI-type glucose analyzers should be used for the analysis of saccharides produced from the hydrolysis of native or pretreated lignocellulosics since the DNS assay is susceptible to interferences and therefore results in inaccurate analyses.
- Research Organization:
- Univ. of Arkansas, Fayetteville
- OSTI ID:
- 6416337
- Journal Information:
- Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 26:7; ISSN BIBIA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
09 BIOMASS FUELS
090222* -- Alcohol Fuels-- Preparation from Wastes or Biomass-- (1976-1989)
140504 -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
CARBOHYDRATES
CELLULOSE
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
CHROMATOGRAPHY
COMPARATIVE EVALUATIONS
DECOMPOSITION
ENZYMATIC HYDROLYSIS
FUNGI
HYDROLYSIS
LIQUID COLUMN CHROMATOGRAPHY
LYSIS
MEASURING METHODS
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SACCHARIFICATION
SEPARATION PROCESSES
SOLVOLYSIS
SPECTROPHOTOMETRY
090222* -- Alcohol Fuels-- Preparation from Wastes or Biomass-- (1976-1989)
140504 -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
CARBOHYDRATES
CELLULOSE
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
CHROMATOGRAPHY
COMPARATIVE EVALUATIONS
DECOMPOSITION
ENZYMATIC HYDROLYSIS
FUNGI
HYDROLYSIS
LIQUID COLUMN CHROMATOGRAPHY
LYSIS
MEASURING METHODS
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SACCHARIFICATION
SEPARATION PROCESSES
SOLVOLYSIS
SPECTROPHOTOMETRY