Bradykinin-activated transmembrane signals are coupled via N/sub o/ or N/sub i/ to production of inositol 1,4,5-trisphosphate, a second messenger in NG108-15 neuroblastoma-glioma hybrid cells

Journal Article · Sat Feb 01 00:00:00 EST 1986 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.83.4.942· OSTI ID:6415710

Higashida, H; Streaty, R A; Klee, W; Nirenberg, M

The addition of bradykinin to NG108-15 cells results in a transient hyperpolarization followed by prolonged cell depolarization. Injection of inositol 1,4,5-trisphosphate or CaS into the cytoplasm of NG108-15 cells also elicits cell hyperpolarization followed by depolarization. Tetraethylammonium ions inhibit the hyperpolarizing response of cells to bradykinin or inositol 1,4,5-trisphosphate. Thus, the hyperpolarizing phase of the cell response may be due to inositol 1,4,5-trisphosphate-dependent release of stored UVCa-labelled CaS into the cytoplasm, which activates CaS -dependent K channels. The depolarizing phase of the cell response to bradykinin is due largely to inhibition of M channels, thereby decreasing the rate of K efflux from cells and, to a lesser extent, to activation of CaS -dependent ion channels and CaS channels. In contrast, injection of inositol 1,4,5-trisphosphate or CaS into the cytosol did not alter M channel activity. Incubation of NG108-15 cells with pertussis toxin inhibits bradykinin-dependent cell hyperpolarization and depolarization. Bradykinin stimulates low K/sub m/ GTPase activity and inhibits adenylate cyclase in NG108-15 membrane preparations but not in membranes prepared from cells treated with pertussis toxin. These results show that (bradykinin-receptor) complexes interact with N/sub o/ or N/sub i/ and suggest that N/sub o/ and/or N/sub i/ mediate the transduction of signals from bradykinin receptors to phospholipase C and adenylate cyclase.

• Research Organization: National Institutes of Health (NIH), Bethesda, MD (United States)
• OSTI ID: 6415710
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 83:4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CALCIUM 45
UPTAKE
MEMBRANE TRANSPORT
CHEMICAL ACTIVATION
TUMOR CELLS
ELECTRIC POTENTIAL
BIOELECTRICITY
BRADYKININ
CALCIUM CHLORIDES
CYCLASES
INOSITOLS
TEAB
TOXINS
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
AMINES
AMMONIUM COMPOUNDS
ANIMAL CELLS
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BROMIDES
BROMINE COMPOUNDS
CALCIUM COMPOUNDS
CALCIUM HALIDES
CALCIUM ISOTOPES
CARBOHYDRATES
CHLORIDES
CHLORINE COMPOUNDS
DAYS LIVING RADIOISOTOPES
ELECTRICITY
ENZYMES
EVEN-ODD NUCLEI
HALIDES
HALOGEN COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOTOPES
KININS
LYASES
MATERIALS
MONOSACCHARIDES
NUCLEI
ORGANIC COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS
QUATERNARY COMPOUNDS
RADIOISOTOPES
SACCHARIDES
TOXIC MATERIALS
550201* - Biochemistry- Tracer Techniques