Effects of cholera toxin and isobutylmethylxanthine on growth of human fibroblasts

Journal Article · Fri Aug 01 04:00:00 EDT 1986 · Am. J. Physiol.; (United States)

OSTI ID:6415479

Espinoza, B; Wharton, W

Cholera toxin produced a dose-dependent decrease in the restimulation of G0/G1 traverse in density-arrested human fibroblasts but did not inhibit the stimulation of cells arrested in G0 after serum starvation at low density. In addition, cholera toxin did not inhibit the proliferation of sparse logarithmically growing human fibroblasts, even when low concentrations of the phosphodiesterase inhibitor isobutylmethylxanthine (IBMX) were also present. However, the final density to which sparse cells grew was limited by cholera toxin, when added either alone or together with low concentrations of IBMX. In contrast, high concentrations of the phosphodiesterase inhibitor alone produced a profound inhibition in the growth of sparse human fibrobasts. IBMX produced an inhibition both in the G1 and in the G2 phases of the cell cycle by a mechanism(s) that was not related to the magnitude of the increases in adenosine 3,5-cyclic monophosphate concentrations.

• Research Organization: Los Alamos National Lab., NM
• DOE Contract Number: W-7405-ENG-36
• OSTI ID: 6415479
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 251:2; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADENOSINE
ANIMAL CELLS
ANTIGENS
AROMATICS
AZAARENES
AZINES
BACTERIAL DISEASES
BIOLOGICAL EFFECTS
CELL CYCLE
CELL FLOW SYSTEMS
CHOLERA
CONNECTIVE TISSUE CELLS
DISEASES
DOSE-RESPONSE RELATIONSHIPS
FIBROBLASTS
GROWTH
HETEROCYCLIC COMPOUNDS
INFECTIOUS DISEASES
LABELLED COMPOUNDS
MATERIALS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PURINES
PYRIMIDINES
RIBOSIDES
SOMATIC CELLS
THYMIDINE
TOXIC MATERIALS
TOXINS
TRITIUM COMPOUNDS
XANTHINES