Nerve injury stimulates the secretion of apolipoprotein E by nonneuronal cells

Journal Article · Sat Feb 01 04:00:00 EST 1986 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.83.4.1130· OSTI ID:6411482

Snipes, G J; McGuire, C B; Norden, J J; Freeman, J A

Nerve trauma initiates significant changes in the composition of proteins secreted by nonneuronal cells. The most prominent of these proteins is a 37-kDa protein, whose expression correlates with the time course of nerve development, degeneration, and regeneration. The authors report that the 37-kDa protein is apolipoprotein E (apoE). They produced a specific antiserum against the 37-kDa protein isolated from previously crushed nerves. This antiserum recognizes a 36-kDa protein in rat serum that they have purified and identified as apoE. The anti-37-kDa antiserum also recognizes apoE on electrophoretic transfer blots of authentic samples of high and very low density lipoproteins. The nerve 37-kDa protein comigrates with apoE by two-dimensional electrophoresis, shares a similar amino acid composition, and reacts with an antiserum against authentic apoE. The purified apoE specifically blocks the immunoprecipitation of (TVS)methionine-labeled 37-kDa protein synthesized by nonneuronal cells. Thus, on the basis of its molecular mass, isoelectric point, amino acid composition, and immunological properties, they conclude that the 37-kDa protein is apoE. They also used light microscopic immunochemistry to localize apoE following nerve injury. They propose that apoE is synthesized by phagocytic cells in response to nerve injury for the purpose of mobilizing lipids produced as a consequence of axon degeneration.

• Research Organization: Vanderbilt Univ. School of Medicine, Nashville, TN
• OSTI ID: 6411482
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 83:4; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMAL CELLS
ANIMALS
APOLIPOPROTEINS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL RECOVERY
BIOLOGICAL REGENERATION
CARBOXYLIC ACIDS
CHEMISTRY
CYTOCHEMISTRY
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTROPHORESIS
EVEN-ODD NUCLEI
INJURIES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPIDS
LIPOPROTEINS
LIPOTROPIC FACTORS
MAMMALS
METHIONINE
MOLECULAR STRUCTURE
NERVE CELLS
NERVES
NERVOUS SYSTEM
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
RADIOISOTOPES
RATS
RECOVERY
RODENTS
SECRETION
SOMATIC CELLS
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES
VERTEBRATES