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Protein kinase C inhibition by sphingoid long-chain bases: effects on secretion in human neutrophils

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6410486
; ; ;

Sphingoid long-chain bases (sphinganine and sphingosine(So)) have recently been shown to inhibit protein kinase C (PK-C) in vitro and to block activation of the oxidative burst in intact neutrophils (PMN) by inhibiting this enzyme. In the present study, the authors have used So to investigate the role of protein kinase C in stimulus-induced secretion of PMN granule contents. Secretion of the specific granule component lactoferrin (Lf) is completely inhibited by pretreatment with So when either PMA or fLMP is used as the secretogogue. Secretion of lysozyme, a component of both the azurophilic and specific granules, is completely inhibited by So when PMA is used, but only 40% inhibited with fMLP. The secretion of the azurophilic granule markers US -glucuronidase and myeloperoxidase was not affected by So regardless of the agonist used. Data indicate that both PK-C-dependent and -independent pathways participate in the neutrophil secretory response.

Research Organization:
Emory Univ. School of Medicine, Atlanta, GA
OSTI ID:
6410486
Report Number(s):
CONF-870644-
Journal Information:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 46:6; ISSN FEPRA
Country of Publication:
United States
Language:
English

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Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL PATHWAYS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CARCINOGENS
ENZYME INHIBITORS
ENZYMES
ESTERS
INHIBITION
KINETICS
LEUKOCYTES
MAMMALS
MAN
MATERIALS
NEUTROPHILS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHENYLALANINE
PHORBOL ESTERS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PRIMATES
REACTION KINETICS
SECRETION
TRANSFERASES
VERTEBRATES