Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Protein kinase C inhibition by sphingoid long-chain bases: effects on secretion in human neutrophils

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6410486
; ; ;
Sphingoid long-chain bases (sphinganine and sphingosine(So)) have recently been shown to inhibit protein kinase C (PK-C) in vitro and to block activation of the oxidative burst in intact neutrophils (PMN) by inhibiting this enzyme. In the present study, the authors have used So to investigate the role of protein kinase C in stimulus-induced secretion of PMN granule contents. Secretion of the specific granule component lactoferrin (Lf) is completely inhibited by pretreatment with So when either PMA or fLMP is used as the secretogogue. Secretion of lysozyme, a component of both the azurophilic and specific granules, is completely inhibited by So when PMA is used, but only 40% inhibited with fMLP. The secretion of the azurophilic granule markers US -glucuronidase and myeloperoxidase was not affected by So regardless of the agonist used. Data indicate that both PK-C-dependent and -independent pathways participate in the neutrophil secretory response.
Research Organization:
Emory Univ. School of Medicine, Atlanta, GA
OSTI ID:
6410486
Report Number(s):
CONF-870644-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
Country of Publication:
United States
Language:
English

Similar Records

Inhibition of the neutrophil oxidative burst by sphingoid long-chain bases: role of protein kinase C in the activation of the burst
Journal Article · Thu May 01 00:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) · OSTI ID:5117441
Free sphingosine in control and stimulated human neutrophils
Conference · Fri May 01 00:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) · OSTI ID:6150476
Inhibition of phorbol ester-induced differentiation of HL-60 cells by long-chain (sphingoid) bases
Conference · Thu May 01 00:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) · OSTI ID:5202858

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL PATHWAYS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CARCINOGENS
ENZYME INHIBITORS
ENZYMES
ESTERS
INHIBITION
KINETICS
LEUKOCYTES
MAMMALS
MAN
MATERIALS
NEUTROPHILS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHENYLALANINE
PHORBOL ESTERS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PRIMATES
REACTION KINETICS
SECRETION
TRANSFERASES
VERTEBRATES