In vitro serum protein-binding characteristics of bis-(2-ethylhexyl) phthalate and its principal metabolite, mono-(2-ethylhexyl) phthalate

Journal Article · Thu Mar 31 23:00:00 EST 1988 · Environ. Health Perspect.; (United States)

DOI:https://doi.org/10.1289/ehp.8877151· OSTI ID:6409338

Griffiths, W C; Camara, P D; Saritelli, A; Gentile, J

The metabolism and toxicity of the ubiquitous plasticizer, bis-(2-ethylhexyl) phthalate (DEHP), and its principal metabolite, mono-(2-ethylhexyl) phthalate (MEHP), have been extensively investigated. In an attempt to understand their disposition in man, the authors studied the in vitro serum protein-binding characteristics of these compounds, using ultracentrifugation and agarose gel electrophoresis. The association of DEHP and lipoproteins was shown to be highly dependent upon, and proportional to, the lipid concentration of the serum. It appears that more than half of the serum DEHP is bound to proteins with density greater than 1.21 g/mL when the concentration of cholesterol is below 300 mg/dL or the cholesterol and triglyceride total concentration is less than 600 mg/dL. As the cholesterol and triglyceride concentrations increase, the percent DEHP bound to VLDL, IDL, and LDL increases. MEHP is bound principally to nonlipoprotein constituents in the serum, and this binding distribution is unaffected by lipid concentration. The percent binding of DEHP and MEHP to individual proteins was also found to be unaffected by their concentrations in serum. These data indicate that the protein-binding characteristics of these compounds, in vitro, is somewhat more complex than previously reported.

• Research Organization: Brown Univ., Providence, RI (USA)
• OSTI ID: 6409338
• Journal Information: Environ. Health Perspect.; (United States), Journal Name: Environ. Health Perspect.; (United States) Vol. 77; ISSN EVHPA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMISTRY
CARBON 14 COMPOUNDS
CARBOXYLIC ACID SALTS
CENTRIFUGATION
CHEMISTRY
CHOLESTEROL
CHROMATOGRAPHY
COUNTING TECHNIQUES
ELECTROPHORESIS
HYDROXY COMPOUNDS
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
METABOLITES
ORGANIC COMPOUNDS
PHTHALATES
PROTEINS
SCINTILLATION COUNTING
SEPARATION PROCESSES
STEROIDS
STEROLS
THIN-LAYER CHROMATOGRAPHY
TRITIUM COMPOUNDS
ULTRACENTRIFUGATION