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Inhibition of phorbol ester-induced polyamine accumulation in mouse epidermis by anti-inflammatory steroid

Journal Article · · Biochem. Biophys. Res. Commun.; (United States)
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Application of the tumor-promoting phorbol diester 12-O-tetradecanoyl-phorbol-13-acetate to mouse epidermis causes a large increase in the activity of ornithine decarboxylase and in polyamine accumulation. Concurrent application of fluocinolone acetonide, an anti-inflammatory steroid that dramatically inhibits tumor promotion, resulted in a dose-dependent decrease in the 12-O-tetradecanoylphorbol-13-acetate-stimulated ornithine decarboxylase activity and the subsequent rise in spermidine levels. Spermine and putrescine levels were not greatly affected by fluocinoline acetonide treatment except that maximal putrescine values occurred later in time. Doses of the glucocorticoid as low as 0.1 ..mu..g inhibited the 12-O-tetradecanoylphorbol-13-acetate-induced ornithine decarboxylase activity by as much as 50% and the rise in spermidine accumulation by 30% after coincident treatment of female Sencar mice.

Research Organization:
Oak Ridge National Lab., TN
DOE Contract Number:
W-7405-ENG-26
OSTI ID:
6407826
Journal Information:
Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 91:4; ISSN BBRCA
Country of Publication:
United States
Language:
English

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Related Subjects

550200 -- Biochemistry
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMAL TISSUES
ANIMALS
BIOCHEMISTRY
BODY
CARCINOGENESIS
CARCINOGENS
CATALYSIS
CHEMISTRY
DOSE-RESPONSE RELATIONSHIPS
EPIDERMIS
EPITHELIUM
ESTERS
INFLAMMATION
MAMMALS
METABOLISM
MICE
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PATHOLOGICAL CHANGES
PHORBOL ESTERS
RESPONSE MODIFYING FACTORS
RODENTS
SKIN
SPERMIDINE
STEROIDS
SYMPTOMS
TISSUES
TOXICITY
VERTEBRATES