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Rapid localization of carbon 14-labeled molecules in biological samples by ion mass microscopy

Journal Article · · J. Histochem. Cytochem.; (United States)
DOI:https://doi.org/10.1177/37.2.2911003· OSTI ID:6407309
; ; ; ;
We report here on the ability of secondary ion mass spectrometry (SIMS) to provide rapid imaging of the intracellular distribution of /sup 14/C-labeled molecules. The validity of this method, using mass discrimination of carbon 14 atoms, was assessed by imaging the distribution of two molecules of well-known metabolism, (/sup 14/C)-thymidine and (/sup 14/C)-uridine, incorporated by human fibroblasts in culture. As expected, /sup 14/C ion images showed the presence of (/sup 14/C)-thymidine in the nucleus of dividing cells, whereas (/sup 14/C)-uridine was present in the cytoplasm as well as the nucleus of all cells, with a large concentration in the nucleoli. The time required to obtain the distribution images with the SMI 300 microscope was less than 6 min, whereas microautoradiography, the classical method for mapping the tissue distribution of /sup 14/C-labeled molecules, usually requires exposure times of several months. Secondary ion mass spectrometry using in situ mass discrimination is proposed here as a very sensitive method which permits rapid imaging of the subcellular distribution of molecules labeled with carbon 14.
Research Organization:
SC 27 de l'INSERM, Creteil (France)
OSTI ID:
6407309
Journal Information:
J. Histochem. Cytochem.; (United States), Journal Name: J. Histochem. Cytochem.; (United States) Vol. 37:2; ISSN JHCYA
Country of Publication:
United States
Language:
English

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Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AZINES
BIOLOGICAL LOCALIZATION
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CARBON 14 COMPOUNDS
CELL CULTURES
CONNECTIVE TISSUE CELLS
DISTRIBUTION
FIBROBLASTS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MAN
MASS SPECTROSCOPY
MATERIALS
MOLECULES
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
PYRIMIDINES
RIBOSIDES
SOMATIC CELLS
SPECTROSCOPY
SUBCELLULAR DISTRIBUTION
THYMIDINE
TRACER TECHNIQUES
URINE
VERTEBRATES
WASTES