Ion microscopy: a new approach for subcellular localization of labelled molecules

Journal Article · Thu Dec 01 04:00:00 EST 1988 · Scan. Microsc.; (United States)

OSTI ID:5990775

Hindie, E; Hallegot, P; Chabala, J M; Thorne, N A; Coulomb, B; Levi-Setti, R; Galle, P

Secondary ion mass spectroscopy (SIMS) was used to obtain images representing the intracellular distribution of molecules labelled with carbon 14. Deoxyadenosine labelled with carbon 14 was added to a cultured human fibroblast cell medium, and the intracellular distribution of this molecule was studied using three different SIMS instruments: the CAMECA IMS 3F and SMI 300 ion microscopes and the UC-HRL scanning ion microprobe. Carbon 14 distribution images obtained by this method show that deoxyadenosine U-C14 is present in the cytoplasm as well as the nucleus, with a higher concentration in the nucleoli. Our study clearly demonstrates that ion microscopy is well suited for carbon 14 detection and localization at the subcellular level, permitting a wide variety of microanalytical tracer experiments.

• Research Organization: Faculte de Medecine, Creteil (France)
• OSTI ID: 5990775
• Journal Information: Scan. Microsc.; (United States), Journal Name: Scan. Microsc.; (United States) Vol. 2:4; ISSN SCMIE
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADENOSINE
ANIMAL CELLS
ANIMALS
CARBON 14 COMPOUNDS
CELL CULTURES
CONNECTIVE TISSUE CELLS
DISTRIBUTION
FIBROBLASTS
LABELLED COMPOUNDS
MAMMALS
MAN
MASS SPECTROSCOPY
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
PRIMATES
RIBOSIDES
SOMATIC CELLS
SPECTROSCOPY
SUBCELLULAR DISTRIBUTION
VERTEBRATES