Toxicity of polycyclic aromatic hydrocarbons. III. Effects of beta-naphthoflavone pretreatment on hepatotoxicity of compounds produced in the ozonation or NO/sub 2/-nitration of phenanthrene and pyrene in rats

Journal Article · Wed Apr 01 04:00:00 EST 1987 · Vet. Hum. Toxicol.; (United States)

OSTI ID:6403660

Yoshikawa, T; Ruhr, L P; Flory, W; Banton, M I; Giamalva, D; Church, D F; Pryor, W A

Male Sprague-Dawley rats were treated ip with beta-naphthoflavone (BNF, 40 mg/kg/day) in dimethylsulfoxide (DMSO, 26.7 mg BNF/ml) for three days. At 24 hr after the pretreatment DMSO (3.0 ml/kg), phenanthrene (150 mg/kg), ozonized or nitrated products of phenanthrene (150 mg/kg), pyrene (150 mg/kg), or ozonized or nitrated products of pyrene (150 mg/kg) were injected ip. Phenanthrene, pyrene, and their ozonized or nitrated products were dissolved in DMSO (50 mg/ml). No increase in the level of aspartate aminotransferase (AST), alanine aminotransferase (ALT) or sorbitol dehydrogenase (SDH) was seen in the pretreated rats 48 hr after the treatment. This is in contrast to what was seen in previous work without the BNF pretreatment. BNF pretreatment induced a small but significant increase in gamma-glutamyl transpeptidase (GGTP) levels. No treatment group receiving BNF differed from another with respect to GGTP. A decrease in lactate dehydrogenase (LDH) levels was noted in the nitro-PAH treatment groups; the same phenomenon was observed earlier in rats treated with nitro-PAH without BNF treatment. These results suggest that the mixed-function oxidase systems specifically induced by BNF have a protective effect against the hepatotoxicity of the oxonized or nitrated products of phenanthrene and pyrene.

• Research Organization: Louisiana Agricultural Experiment Station, Baton Rouge
• OSTI ID: 6403660
• Journal Information: Vet. Hum. Toxicol.; (United States), Journal Name: Vet. Hum. Toxicol.; (United States) Vol. 2; ISSN VHTOD
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINOTRANSFERASES
ANIMALS
AROMATICS
BIOLOGICAL EFFECTS
CHALCOGENIDES
CONDENSED AROMATICS
DMSO
ENZYMES
FLAVENOIDS
FLAVONES
HEMIACETAL DEHYDROGENASES
HYDROCARBONS
HYDROLASES
LACTATE DEHYDROGENASE
MAMMALS
MIXED-FUNCTION OXIDASES
NITRO COMPOUNDS
NITROGEN COMPOUNDS
NITROGEN DIOXIDE
NITROGEN OXIDES
NITROGEN TRANSFERASES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
OXYGENASES
OZONE
PEPTIDE HYDROLASES
PHENANTHRENE
PYRENE
RATS
RODENTS
SULFOXIDES
TOXICITY
TRANSFERASES
VERTEBRATES