Effects of salt on the pattern of protein synthesis in barley roots
The effect of salt stress on the incorporation of (/sup 3//sub 5/S)methionine into protein was examined in roots of barley (Hordeum vulgare L.cv California Mariout 72). Plants were grown in nutrient solution with or without 200 millimolar NaCl. Roots of intact plants were labeled in vivo and proteins were extracted and analyzed by fluorography of two-dimensional gels. Although the protein patterns for control and salt-stressed plants were qualitatively similar, the net synthesis of a number of proteins was quantitatively changed. The most striking change was a significant increase of label in two protein pairs that had pls of approximately 6.3 and 6.5. Each pair consisted of proteins of approximately 26 and 27 kilodaltons (kD). In roots of control plants, the 27-kD proteins were more heavily labeled in the microsomal fraction relative to the 26-kD proteins, whereas the 26-kD proteins were enriched in the post 178,000g supernatant fraction; in roots of salt treated plants, the 26- and 27-kD proteins were more intensely labeled in both fractions. Labeling of the 26- and 27-kD proteins returned to control levels when salt-stressed plants were transferred to nutrient solution without NaCl. No cross-reaction was detected between the antibody to the 26-kD protein from salt-adapted tobacco cells and the 26- and 27-kD proteins of barley.
- Research Organization:
- Dept. of Agriculture, Albany, CA
- OSTI ID:
- 6401474
- Journal Information:
- Plant Physiol.; (United States), Vol. 83:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
BARLEY
BIOLOGICAL STRESS
METHIONINE
METABOLISM
PROTEINS
BIOSYNTHESIS
LABELLING
SODIUM CHLORIDES
BIOLOGICAL EFFECTS
ELECTROPHORESIS
ROOTS
SULFUR 35
TRACER TECHNIQUES
ALKALI METAL COMPOUNDS
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CEREALS
CHLORIDES
CHLORINE COMPOUNDS
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
GRASS
HALIDES
HALOGEN COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PLANTS
RADIOISOTOPES
SODIUM COMPOUNDS
SULFUR ISOTOPES
SYNTHESIS
560300* - Chemicals Metabolism & Toxicology
550201 - Biochemistry- Tracer Techniques