Potassium-dependent changes in the expression of membrane-associated proteins in barley roots

Journal Article · Sun Apr 01 00:00:00 EST 1990 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.92.4.1128· OSTI ID:5923338

Fernando, M; Kulpa, J; Siddiqi, M Y; Glass, A D.M. ^[1]

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1. Univ. of British Columbia, Vancouver (Canada)

Barley (Hordeum vulgare L. cv Halcyon) seedlings which has been grown in full strength complete inorganic nutrient media (containing 6 millimolar K{sup +}) had high internal K{sup +} concentrations and low values of K{sup +} ({sup 86}Rb{sup +}) influx when influx was measured from solutions containing 100 micromolar K{sup +}. Transfer of these plants to solutions lacking K{sup +} resulted in significant reductions of root and shoot K{sup +} concentrations and values of K{sup +} ({sup 86}Rb{sup +}) influx increased by greater than 10-fold within 3 days. When plants treated in this way were returned to complete solutions, containing K{sup +}, the changes induced by K{sup +} deprivation were reversed. Parallel studies of microsomal membranes by means of SDS-PAGE demonstrated that the expression of a group of polypeptides increased or decreased in parallel with changes of K{sup +} ({sup 86}Rb{sup +}) influx. Most prominent of these were 45 and 34 kilodalton polypeptides which specifically responded to K{sup +} status of the barley plants; their expression was not enhanced by N or P deprivation. The 45 kilodalton polypeptide was susceptible to degradation by a membrane associated protease when microsomes were washing in buffer containing 0.2 millimolar PMSF. This loss was prevented by increasing PMSF concentration to 2 millimolar.

• OSTI ID: 5923338
• Journal Information: Plant Physiology; (USA), Vol. 92:4; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
POTASSIUM
BIOLOGICAL EFFECTS
BARLEY
MEMBRANE PROTEINS
POLYPEPTIDES
ROOTS
RUBIDIUM 86
RUBIDIUM COMPOUNDS
SEEDLINGS
TRACER TECHNIQUES
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
ALKALI METALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CEREALS
DAYS LIVING RADIOISOTOPES
ELEMENTS
GRASS
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LILIOPSIDA
MAGNOLIOPHYTA
METALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PEPTIDES
PLANTS
PROTEINS
RADIOISOTOPES
RUBIDIUM ISOTOPES
550201* - Biochemistry- Tracer Techniques